Egr-1基因剔除减少炎性相关因子在实验性胰腺炎中的表达  

Reduction of inflammatory-related factor expression in experimental acute pancreatitis in Egr-1 knockout mice

作　　者：段佑才[1] 姜泊[2] 马高峰[2] 徐智民[2] 陈晓文[2] 程天明[2] 戴益琛[2] 陈学清[2] 

机构地区：[1]广东省第二人民医院内科,广东广州510317 [2]广州南方医院消化内科,广东广州510515

出　　处：《中国病理生理杂志》2007年第4期739-743,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370648)

摘　　要：目的:探讨Egr-1基因剔除对实验性胰腺炎小鼠胰腺组织中炎性相关因子表达的影响。方法:利用Egr-1基因剔除小鼠,采用大剂量雨蛙素诱导的实验性胰腺炎模型,观察Egr-1基因剔除后,胰腺组织水肿、MPO水平、血清淀粉酶水平、肺组织MPO水平的改变。并利用定量PCR的方法,检测胰腺组织中炎症相关因子组织因子(TF)、纤维蛋白溶酶原激活因子抑制因子(PAI-1)、单核细胞趋化吸引蛋白1(MCP-1)、Gro-1、IL-6和细胞间黏附分子-1(ICAM-1)mRNA的表达。结果:Egr-1基因剔除小鼠胰腺组织水肿明显轻于野生型,但组织MPO水平与血清淀粉酶与野生型组间相比无明显差异;肺组织MPO水平明显低于野生型。定量PCR检测结果表明,Egr-1基因剔除组,胰腺组织中TF、PAI-1,以及MCP-1、ICAM-1和IL-6 mRNA的表达,明显少于野生型组。结论:Egr-1基因剔除可明显减轻急性胰腺炎的严重程度,其作用可能通过减少胰腺组织中TF、PAI-1,以及MCP-1、ICAM-1和IL-6的表达而实现。AIM： To observe the effects of Egr - 1 gene knockout on the expression of inflammatory - related factors in pancreatic tissue in a mouse acute pancreatitis model. METHODS： The experimental pancreatitis was induced by high - dose of cearulein in wildtype mice and Egr - 1 knockout mice. The pancreatitis indexes, such as serum amylase, pancreata edema, and myeloperoxidase（MPO） levels in pancreata and lungs were recorded. The mRNA levels of tissue factor（ TF）, plasminogen activator inhibitor（ PAI -1 ）, monocyte chemoattractant protein（ MCP- 1 ）, Gro -1, IL- 6 and ICAM - 1 were measured by quantitative PCR. RESULTS： Contrary to wildtype mice, typical pancreatitis was not induced by high - dose cearulein in the Egr - 1 knockout mice, not only markedly reduced edema in pancreata and lungs, but decreased MPO levels in lungs as well were found. Furthermore, the mRNA of TF, PAI, MCAP, ICAM- 1 and IL-6 in pancreata were significantly decreased in Egr - 1 knockout mice. CONCLUSION： The severity of pancreatitis and lung damage is ameliorated in Egr - 1 knockout mice stimulated by high - dosage of cearulein, which was probably mediated by decreasing expression of inflammatory - related factors in pancreata, such as TF, PAI, MCP - 1, ICAM - 1 and IL - 6.

关 键 词：胰腺炎 早期生长反应因子 基因 Egr-1 基因敲除 趋化因子 巨噬细胞 

分 类 号：R363[医药卫生—病理学]