应激状态下大鼠胃壁细胞H^+-K^+-ATP酶活性测定及电镜酶细胞化学研究  

Activity of H^+-K^+-ATPase in gastric parietal cells under stress in rats and analysis of electron microscopic enzyme cytochemistry

作　　者：李玉梅[1] 陆国明[2] 邹晓平[3] 李兆申[3] 谢志芳[4] 

机构地区：[1]武警浙江省总队医院消化内科,浙江嘉兴314000 [2]嘉兴学院医学院,浙江嘉兴314001 [3]第二军医大学长海医院消化内科,上海200433 [4]第二军医大学细胞生物学教研室,上海200433

出　　处：《中国病理生理杂志》2007年第4期753-756,共4页Chinese Journal of Pathophysiology

基　　金：全军"十五"医药卫生科研基金重点资助项目(No.01Z059);浙江省嘉兴市科技计划资助项目(No.20031061)

摘　　要：目的:探讨应激状态下大鼠胃壁细胞H+-K+-ATP酶活性及电镜酶细胞化学染色的变化。方法:将24只SD大鼠随机分为对照组、应激组和应激+奥美拉唑(OM)组,采用水浸-束缚应激(WRS)动物模型,检测胃粘膜溃疡指数(UI)和壁细胞H+-K+-ATP酶活性,观察壁细胞超微结构变化及H+-K+-ATP酶细胞化学染色结果。结果:应激组胃粘膜UI和壁细胞H+-K+-ATP酶活性明显高于对照组(P<0.01和P<0.05),而应激+OM组UI和H+-K+-ATP酶活性明显低于应激组(P<0.01);电镜下对照组壁细胞呈静息状态,应激组壁细胞内分泌小管密集呈激活状态,而应激+OM组分泌小管明显扩张,绒毛稀少;酶细胞化学染色显示对照组壁细胞的分泌小管和顶部质膜有少量黑色点状酶反应产物沉积,应激组壁细胞的分泌小管可见多量的、密集分布的黑色点状酶反应产物,而应激+OM组分泌小管上几乎无反应产物沉积。结论:应激状态下大鼠胃壁细胞H+-K+-ATP酶活性升高,且与壁细胞超微结构改变相一致,提示胃酸是应激性溃疡发生的重要因素之一。AIM： To demonstrate the changes of activity and electron microscopic enzyme cytochemistry staining of H^＋ - K^＋ - ATPase of gastric parietal cells under stress in rats. METHODS ： Twenty - four male SD rats were randomly divided into normal group, stress group and stress ＋ omeprazole （OM） group. Water immersion - restraint stress （WRS） model in SD rats was performed. The ulcer index （UI） of gastric mucosa and H^＋ - K^＋ - ATPase activity of gastric parietal cells were measured. The changes of ultrastructure and electron microscopic enzyme cytochemistry staining of parietal cells were observed under transmission electron microscope （TEM）. RESULTS： Compared with control group, the UI of gastric mucosa and H^＋ - K^＋ -ATPase activity of gastric parietal cells increased （P 〈 0. 01 and P 〈 0. 05 ） in stress group. In stress ＋ OM group, both UI and H^＋ - K^＋ - ATPase activity decreased （ P 〈 0. 01 ） compared with stress group. Parietal cells were in a resting state in control group, and became active in stress group, where plenty of intracellular canaliculi were observed under the TEM. In stress ＋ OM group, the dilated intracellular canaliculi lined with rare microvilli were founded. Enzyme cytochemistry staining showed that there was little black punctate enzyme reactive product scatted in intracellular canaliculi and the apical plasma membrane of parietal cells in control group, and there were large amounts of black enzyme reactive product accumulated at the intracellular canaliculi in stress group. Scarcely deposition of enzyme reactive product in intracellular canaliculi was observed in stress ＋ OM group. CONCLUSION： The results indicate that the H^＋ - K^＋ - ATPase activity of gastric parietal cells increases under WRS, and is in accordance with uhrastructure changes. These findings suggeste that gastric acid might be one of the most important factors that result in stress ulcer.

关 键 词：应激 溃疡 壁细胞 胃 H^+-K^+-ATP酶 

分 类 号：R363[医药卫生—病理学]