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作 者:程燕[1] 叶文才[1] 石俊敏[1] 张坤[1] 吕艳青[1] 姚新生[1] 栗原博[1]
机构地区:[1]暨南大学中药及天然药物研究所,广东广州510632
出 处:《中国病理生理杂志》2007年第4期772-775,共4页Chinese Journal of Pathophysiology
基 金:广东省关键领域重点突破项目资助(No.2003A30909);广州市重点科技攻关项目资助(No.2004Z2-E5021)
摘 要:目的:研究23-羟基白桦酸诱导人结肠癌细胞株LoVo细胞凋亡过程中活性氧(ROS)的变化及其作用机制。方法:光学显微镜法观察23-羟基白桦酸作用LoVo细胞后细胞形态学改变,并采用流式细胞仪检测细胞凋亡及细胞内ROS水平。结果:分别以浓度为25、50、100、200μmol/L 23-羟基白桦酸作用LoVo细胞48h后,在光学显微镜下观察到明显的凋亡细胞形态学改变;经流式细胞仪检测,细胞凋亡率分别为(7.17±2.31)%、(15.60±4.02)%、(32.47±5.25)%及(52.71±5.93)%,呈现一定的浓度依赖性关系。23-羟基白桦酸可使LoVo细胞内ROS的水平明显高于空白对照组。细胞内ROS含量(MFI,平均荧光强度)分别为2.83±0.80、5.97±1.72、12.53±2.57及16.73±4.58。其中,浓度为100和200μmol/L 23-羟基白桦酸的作用较明显,与对照组(2.13±0.32)比有显著差异(P<0.05)。结论:23-羟基白桦酸具有明显的诱导LoVo细胞凋亡作用,其作用机制可能与23-羟基白桦酸引起细胞内ROS水平增加有关。AIM: To investigate the changes of reactive oxygen species (ROS) in apoptosis of LoVo cells induced by 23 - hydroxybetulinic acid. METHODS : LoVo cells were treated with 23 - hydroxybetulinic acid. The apoptotic morphological change was observed under the light microscope. Intracellular ROS production and the rate of apoptosis were detected by flow cytometry. RESULTS : LoVo cells improved apoptotic morphological changes treated with 23 - hydroxybetulinic acid for 48 h. At concentrations of 25, 50, 100, 200 μmol/L of 23 - hydroxybetulinic acid, the apoptotic rates of LoVo cells were (7.17 ± 2. 31 ) %, ( 15.60 ± 4. 02) %, ( 32. 47 ± 5.25 ) % and (52. 71 ± 5.93) %, respectively. The results indicated a certain concentration - dependent relationship. 23 - hydroxybetulinic acid caused an increase in the ROS production, and the ROS levels were 2.83 ± 0. 80, 5.97 ± 1.72, 12. 53 ± 2. 57 and 16. 73 ± 4. 58. Compared with the control group (2. 13 ±0. 32), the increase in ROS production in LoVo cells at the concentration of 100, 200 μmol/L of 23 - hydroxybetulinic acid treatment was significant ( P 〈 0. 05 ). CONCLUSION: 23 - hydroxybetulinic acid induces LoVo cell apoptosis. The production of ROS may play a crucial role in the process of the LoVo cell apoptosis.
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