CCK-8上调小鼠腹腔巨噬细胞B7.1和B7.2表达并增强其协同刺激活性  

CCK-8 upregulates B7.1 and B7.2 expressions and enhances the costimulatory activity of murine peritoneal macrophages

作　　者：张风华[1] 李淑瑾[1] 丛斌[1] 张正茂[2] 朱桂军[2] 马春玲[1] 丛军 刘宁[1] 倪志宇[1] 付丽红[1] 

机构地区：[1]河北医科大学法医系,河北石家庄050017 [2]河北医科大学第四医院,河北石家庄050011 [3]河北省赵县人民医院,河北赵县051530

出　　处：《中国病理生理杂志》2007年第4期776-779,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30500193)

摘　　要：目的:探讨八肽胆囊收缩素(CCK-8)对静息巨噬细胞B7.1和B7.2表达及其协同刺激功能的影响。方法:用CCK-8(10-12-10-6mol/L)孵育小鼠腹腔巨噬细胞一定时间,采用流式细胞术分析细胞表面B7.1和B7.2含量的变化。用免疫磁珠从小鼠脾细胞分离CD4+T细胞,按4∶1数量比与腹腔巨噬细胞(预先用CCK-8和/或抗B7.1抗体、抗B7.2抗体、CCK1R拮抗剂CR1409、CCK2R拮抗剂CR2945孵育24 h)共同体外培养,同时加入ConA 5 mg/L,采用[3H]掺入法测定CD4+T细胞增殖反映巨噬细胞的协同刺激活性。结果:CCK-8可上调静息巨噬细胞B7.1及B7.2的表达,并增强巨噬细胞的协同刺激活性。CCK-8的作用呈剂量依赖性,最大效应剂量是在10-9-10-7mol/L之间。抗B7.2抗体可减轻CCK-8增强巨噬细胞协同刺激活性的作用,CR1409及CR2945均能逆转CCK-8的上述作用,且CR1409的作用较CR2945更明显。结论:CCK-8通过上调巨噬细胞B7.2表达而增强其协同刺激活性,该作用由CCK1R及CCK2R介导,其中CCK1R起主要介导作用。AIM： To investigate in vitro effects of cholecystokinin octapeptide（ CCK -8） on the expressions of B7. 1 and B7.2 and the costimulatory activity of T lymphocytes in unstimulated macrophages. METHODS ： Mouse peritoneal macrophages were isolated and incubated with CCK -8（ 10^-12 - 10^-6 mol/L） for indicated time. The B7. 1 and B7. 2 expressions of murine peritoneal macrophages were analyzed by flow cytometry. CD4^＋ T cells were isolated from mouse spleen using immunomagnetic beads, and cultured with 1/4 numbers of macrophages which were pretreated with CCK- 8 and/or anti - B7.1 antibody, anti - B7.2 antibody, CCK1R antagonist CR1409, CCK2R antagonist CR2945 for 24 h. ConA was added into the culture medium to stimulate CD4^＋T cell proliferation. The proliferation was determined by measuring [^3H] - TdR incorporation in a 13 - scintillation counter. RESULTS ： B7.1 and B7.2 expressions and costimulatory activity of peritoneal macrophages were enhanced by CCK -8 in a dose -dependent manner, and the maximal effects occurred at the concentrations of 10^-9 mol/L to 10^-7 mol/L. Anti - B7. 2 antibody, but not anti - B7. 1 antibody, reduced the modulatory role of CCK - 8 on costimulatory activity. Both CR1409 and CR2945 reversed the effect of CCK - 8 on costimulation, and the role of CR1409 was more significant. CONCLUSION ： CCK -8 enhances macrophage costimulatory activity by upregulating B7.2 expression, which is mediated by CCK1R and CCK2R. CCK1R might be the major receptor responsible for the modulation of CCK - 8 on costimulation.

关 键 词：巨噬细胞 胆囊收缩素 

分 类 号：R363.2[医药卫生—病理学]