子宫内膜癌hCG异位自分泌的意义及对COX-2的调节作用  

Ectopic hCG in endometrial carcinoma and its effect on COX-2 expre-ssion

作　　者：郑军生[1] 林琛莅[1] 钟雪云[1] 

机构地区：[1]暨南大学医学院病理教研室

出　　处：《中国病理生理杂志》2007年第4期799-802,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30670795);广东省科技厅科技计划资助项目(No.2004B31081008)

摘　　要：目的:探讨hCG对JEC子宫内膜癌细胞中COX-2表达的调节,以期进一步阐明恶性肿瘤hCG异位自分泌的意义。方法:放射免疫定量检测JEC子宫内膜癌细胞株β-hCG的自分泌;MTT实验检测hCG对JEC细胞活性的影响和hCG增加选择性COX-2抑制剂NS398对JEC细胞的抑制作用;Western blotting分析hCG对JEC细胞COX-2蛋白表达的影响。结果:JEC子宫内膜癌细胞株存在β-hCG的自分泌;hCG可增加JEC细胞的活性,上调COX-2蛋白的表达,增加选择性COX-2抑制剂NS398对JEC细胞的抑制作用。结论:JEC细胞hCG的异位自分泌,有促进自身生长的作用,也是返祖、去分化的一种表现,上调COX-2蛋白的表达可能是其作用机制之一。AIM ： To investigate the effect of human chorionic gonadotropin （hCG） on cyclooxygenase - 2 （ COX - 2） expression in endometrial carcinoma so as to study the function of ectopic hCG. METHODS ： The ectopic β - hCG in JEC endometrial carcinoma cell lines was quantified by radioimmunoassay. By MTT assay, the effect of hCG on the cell viability of JEC cell lines and the inhibition of selective COX - 2 inhibitor NS398 on JEC cell lines were examined, The effect of hCG on COX - 2 expression was detected by Western blotting. RESULTS ： The ectopic β - hCG release from cultured JEC cell lines were observed, HCG promoted the cell viability, upregulated the expression of COX - 2 protein and increased the inhibition of selective COX - 2 inhibitor NS398 in JEC cell lines. CONCLUSION： The ectopic hCG in JEC endometrial carcinoma cell lines increases cell proliferation, which may be mediated by upregulating the expression of COX - 2 protein.

关 键 词：人绒毛膜促性腺激素 环氧合酶-2 环氧合酶抑制剂 子宫肿瘤 JEC cells 

分 类 号：R363[医药卫生—病理学]