榄香烯复合瘤苗HSP70-肿瘤肽活性成分的纯化及鉴定  

Purification and identification of the active component on HSP70-tumor peptide extracted from elemene combo-tumor cell vaccine

作　　者：沈洁[1] 朴花[2] 于丽华[3] 郭连英[3] 施广霞[3] 钱振超[3] 

机构地区：[1]大连医科大学形态学实验室,辽宁大连116027 [2]大连医科大学生理学教研室,辽宁大连116027 [3]大连医科大学肿瘤生物治疗研究所,辽宁大连116027

出　　处：《大连医科大学学报》2007年第2期97-99,共3页Journal of Dalian Medical University

基　　金：国家自然科学基金重点项目资助(No.39730440-Ⅱ)

摘　　要：[目的]探讨榄香烯复合瘤苗HSP70-肿瘤肽活性成分的纯化方法。[方法]以小鼠HCa-F肝癌和L615白血病细胞为肿瘤模型,分别制备两种榄香烯复合瘤苗,通过亲和层析法制备瘤苗的HSP70-肿瘤肽活性成分,免疫印迹法鉴定两种HSP70-肿瘤肽及其活性。[结果]两种HSP70-肿瘤肽样品均在70 kD附近呈现清晰的一条HSP70-肿瘤肽蛋白带;从HCa-F瘤苗中获得的HSP70-肿瘤肽产量为(2.34±0.28)mg/109个细胞,该产量约是等细胞数L615瘤苗产量的2倍。[结论]该方法适于HSP70-肿瘤肽活性成分的提取并能保持HSP70-肿瘤肽分子的免疫活性。[ Objective] To investigate a purified method of the active component on HSP70 -tumor peptide extracted from Elemene Combo -Tumor cell vaccine （EC -TCV）. [ Methods] The two of EC -TCV were prepared with cells of HCa -F hepatoma and 1-615 leukemia in mice respectively, then the active components on HSP70 -tumor peptide were extracted from the EC -TCV with affinity chromato - graphy, and HSP70 -tumor peptide and their activities were identified by Western blotting. [ Results ] There was a clear bend near 70 kD in each sample of HSP70- tumor peptide; the output of HSP70- tumor peptide extracted from 1 x 109HCa- F cells was 2.34 ＋0.28 mg ,the output is higher than the yield from the equal L615 by twice. [ Conclusion] The method is a suitable for the extraction of HSP70 - tumor peptide and preservation of the activity of HSP70 - tumor peptide.

关 键 词：榄香烯复合瘤苗 HSP70-肿瘤肽 亲和层析法 

分 类 号：R73-3[医药卫生—肿瘤]