昆明山海棠中具有免疫抑制活性的二萜化合物  

Diterpenoids possessed immunosuppressive activity from Tripterygium hypoglaucum

作　　者：张彦文[1] 范云双[2] 王晓东[2] 高文远[2] 段宏泉[1] 

机构地区：[1]天津医科大学药学院,天津300070 [2]天津大学药物与科学技术学院,天津300072

出　　处：《中草药》2007年第4期493-496,共4页Chinese Traditional and Herbal Drugs

基　　金：天津医科大学校重点学科建设项目资助(2004xk32

摘　　要：目的研究雷公藤属植物昆明山海棠的免疫抑制活性成分。方法运用各种硅胶柱色谱、凝胶渗透色谱以及制备高效液相色谱等手段对昆明山海棠氯仿提取物进行分离纯化，以各种有机波谱法鉴定化学单体结构，并通过体外淋巴细胞转化实验进行化学成分的免疫抑制活性研究。结果从中分离鉴定出6个二萜类化合物，分别为雷酚萜酸（triptobenzene H，Ⅰ）、雷藤二萜醌A（triptoquinone A，Ⅱ）、雷藤二萜醌B（triptoquinone B，Ⅲ）、雷藤二萜醌H（triptoquinone H，Ⅳ）、雷酚萜醇（triptonediol，Ⅴ）、雷酚萜（triptonoterpene，Ⅵ）。结论化合物Ⅰ～Ⅲ为首次从该植物中分离得到，化合物Ⅰ～Ⅵ均具有较强的免疫抑制活性。Objective To study the diterpenoids possessed immunosuppressive activity from Tripterygiurn hypoglaucurn. Methods The chemical constituents were isolated from the CHCl3 extracts of T. hypoglaucurn by repeated column chromatography, including silica gel, Sephadex LH-20, and preparative HPLC. Their structures were elucidated on the basis of spectroscopic studies. The immunosuppressive activity of these compounds was tested using the lymphocyte transformation test. Results Compounds Ⅰ - Ⅵ were identified as triptobenzene H （ Ⅰ ）, triptoquinone A （ Ⅱ ）, triptoquinone B （ Ⅲ ）, triptoquinone H （Ⅳ）, triptonediol （Ⅴ）, triptonoterpene （Ⅵ）. Conclusion Compound Ⅰ - Ⅲ are isolated from this medicinal plant for the first time and all the compounds show the significant immunosuppressive activity.

关 键 词：昆明山海棠 根心部 二萜类化合物 免疫抑制活性 

分 类 号：R284.1[医药卫生—中药学]