Developmental Changes of Col3a1 mRNA Expression in Muscle and Their Association with Intramuscular Collagen in Pigs  

猪肌肉组织Col3a1基因表达的发育性变化及其对肌内胶原特性的影响(英文)

作　　者：包新见[1] 曾勇庆[1] 魏述东[2] 王刚[1] 刘婵娟[1] 孙延晓[1] 陈其美[1] 李华[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省莱芜市畜牧兽医技术推广中心,莱芜271100

出　　处：《Journal of Genetics and Genomics》2007年第3期223-228,共6页遗传学报（英文版）

基　　金：This work was supported by the National Animal Breed Resource Preserve Project (No.200014); the Key Project of Shandong Science and Technology Development (No. 20059913); the Sciences Innovation Foundation of Shandong Agricultural University.

摘　　要：Eighty-four castrated boars including Laiwu Black （LW） （weight 30-90 kg, n = 6） and Lulai Black （LL） （weight 40-100 kg, n = 6） were used to study the developmental changes of collagen type Ⅲ alpha 1 （Col3al） mRNA expression in the muscle and their association with intramuscular collagen （IMC）. The muscle total RNA was extracted to determine the abundance of Col3al mRNA using relative quantitative RT-PCR with β-actin mRNA as the internal standard. The results indicated that the developmental patterns of muscle Col3al mRNA in LW and LL pigs were similar. The abundance of Col3al mRNA increased with body weight, but decreased a little at 70 kg and 80 kg phases for LW and LL, respectively. On the whole, the expression level of Col3al mRNA in muscle of LW was higher than that of LL （P 〈 0.05）. Correlation analysis showed that the expression of Col3al mRNA in muscle was positively correlated with total and insoluble IMC, but was negatively correlated with IMC solubility for LW pigs （P 〈 0.01） and LL pigs （P 〈 0.05）, respectively. These results suggest that the muscle Col3al gene expression is affected by body weight and genotype and has important effect on IMC content and characteristics.以30—90妇体重莱芜猪和40—100kg体重鲁莱黑猪共84头去势公猪为试验对象（每组6头），采用相对定量RT-PCR方法，以β-actin作为内标，研究肌肉中编码Ⅲ型胶原的Col3al基因表达的发育性变化及其对肌肉中胶原蛋白含量和性质（溶解度）的影响。结果表明：研究的两个品种猪肌肉中Col3al基因表达的发育性变化基本一致，即随体重的增加，肌肉中Col3al mRNA表达呈逐渐增加趋势，但莱芜猪和鲁莱黑猪分别在70妇和80妇体重组表达量略有下降。总体上莱芜猪肌肉组织Col3al mRNA表达丰度显著高于鲁莱黑猪（P〈0．05）。相关分析表明，莱芜猪肌肉组织Col3al mRNA表达的发育性变化与总胶原和不溶性胶原含量呈极显著正相关（P〈0．01），与胶原溶解度呈极显著负相关（P〈0．01）。鲁莱黑猪肌肉组织Col3al mRNA表达的发育性变化与不溶性胶原和胶原溶解度分别呈显著正相关和负相关妒〈0．05）。研究结果提示：猪肌肉组织中Col3al基因表达具有明显的体重发育和品种特征，其mRNA表达对于肌内胶原的含量和性质有重要影响。

关 键 词：PIG Laiwu Black intramuscular collagen Col3al gene development RT-PCR 

分 类 号：Q953[生物学—动物学]