电化学对人宫颈癌细胞系Hela-S生物学作用的实验研究  

Experiment study of Biological action of Electrochemistry on Human cervical carcinoma Hela-S3 colon

作　　者：唐步坚[1] 李力[1] 赵恒毅[1] 古明华[1] 陈心秋[1] 黄玲莎[1] 唐东平[1] 

机构地区：[1]广西医科大学附属肿瘤医院,南宁530021

出　　处：《肿瘤防治研究》1997年第1期4-7,共4页Cancer Research on Prevention and Treatment

摘　　要：本实验采用体外实验并利用MTT法和放射自影技术，观察在不同电压和电量组合的ECT作用下对人宫颈癌细胞系（Hela）的杀伤率、细胞的增殖曲线、核分裂指数以及细胞增殖周期影响，旨在了解ECT是否对Hela细胞的生物学行为有影响。其结果显示：（1）ECT对Hela细胞的增殖率有明显的抑制作用，其中以5V10C组的抑制率最为明显。（2）ECT对Hela细胞有较强的杀伤作用，而且这种杀伤作用，在电压一定时，与电量成正比。（3）ECT对Hela细胞的增殖周期有明显的影响，这对揭示ECT治癌机理和为临床选择适当有效的治疗参数均有重要的意义。The effect of ECT combinated by different voltage and electric quantity on killer rate of Human cervical carcinoma Hela-S3 colon. proliferating rate of cells, karyokinesis inde and cells proliferating period was observed by MTT methed and autoradiography. The purpose of the experiments is to find out the effect of ECT on the bilological act of Hela-S3 colon. The results showed ECT has the following action: (1) Obvious inhibition action on the proliferating of Hela-S3 colon. The inhibiting rate of 5V10C is the most obvious one. (2) Killer action on Hela-S3 colon. It has direct rate with electric quatity under the same violtage. (3) Obvious effect on the cells proliferating period, mainly on G1-S period and M period is observed, and it is related with voltage, electric quantity. The above results showed ECT has obvious effect on the bilogioal behavior.The results also contribute to the treatment of human cervical carcinoma, they play an important part when doctors are selecting effective ways of treating patients.

关 键 词：电化学治疗 子宫颈癌 HELA细胞 细胞动力学 

分 类 号：R737.330.5[医药卫生—肿瘤] R730.53[医药卫生—临床医学]