内蒙古地区宫颈癌患者人乳头瘤病毒16感染分型及其人类白细胞抗原-DRB1、人类白细胞抗原-DQB1基因多态性的关系  

Study on the association between HPV16-positive cervical cancer and polymorphism of HLA-DRB1,HLA-DQB1 in Inner Mongolian women

作　　者：谢静[1] 王琳[2] 王毅[2] 马旭[2] 乔惠珍[3] 

机构地区：[1]内蒙古自治区医院妇产科,呼和浩特010017 [2]国家人口计生委科学技术研究所,北京100081 [3]内蒙古医学院附属医院妇产科,呼和浩特010050

出　　处：《生殖医学杂志》2007年第2期91-95,共5页Journal of Reproductive Medicine

摘　　要：目的探讨人乳头瘤病毒（HPV）16感染与宿主人类白细胞抗原（HLA）-DRB1、HLA—DQB1等位基因和宫颈癌发生的相关性。方法以聚合酶链反应（PCR）和多重PCR法检测30例宫颈癌、38例正常宫颈组织HPV感染及型别分布；以PCR-SSP法对22例HPV16阳性宫颈癌及35例HPV阴性正常宫颈组织进行HLA-DRB1、HLA—DQB1基因分型。结果HPV16阳性宫颈癌组与HPV阴性正常对照组比较，HLA—DRB1＊15、HLA—DQB1＊0301／9，10、HLA—DQB1＊06基因表型频率显著增加（P〈0.05），经统计学校正后，仅HLA—DRB1＊15、HLA—DQB1＊06等位基因与HPV16阳性宫颈癌之间呈正相关（分别为OR=3．61，95％CI=1．03～13．08；OR=3．63，95％CI=1．03～13．23）。结论HLA—DRB1＊15、HLA—DQB1＊06等位基因增加了HPV16阳性妇女患宫颈癌的危险性。Objective： To study the association between cervical cancer, HPV16 infection and HLA-DRB1, HLA-DQB1 alleles. Methods. PCR and multiplex PCR were used to test the frequency and type distribution of HPV infection in 38 normal cervical tissues and 30 cervical cancer tissues. Sequence-specific primer PCR was adopted to distinguish HLA-DRB1 and HLA-DQB1 genotypes in 35 HPV-negative normal cervical tissues and 22 HPV16-positive cervical cancer tissues. Results： Compared with HPV-negative normal controls, the patients with HPV16-positive cervical cancer had a significantly increased frequency of HLA-DRB1＊ 15, HLA-DQB1 ＊ 0301/9,10; and HLA- DQB1 ＊ 06 alleles （P〈0. 05）. After correction for multiple testing, only the DQB1 ＊ 06 and the DRB1 ＊ 15 alleles remained positively correlated with HPV16-positive cervical cancer patients（OR=3. 61, 95% CI= 1.03-13.08 and OR-=3.63, 95% CI-=1. 03-13.23, respectively）. Conclusion. HLA-DRB1 ＊ 15, HLA-DQB1 ＊ 06 alleles increased the risk of HPV16-positive women in developing cervical cancer.

关 键 词：人乳头瘤病毒 人类白细胞抗原 宫颈癌 

分 类 号：R711.74[医药卫生—妇产科学]