mdr-1基因转移可提高肺癌细胞对阿霉素的抗性  

Fully Length MDR1 cDNA Transfer Conferring Resistance to Adriamycine on Sensitive Cells GLC

作　　者：周中军[1] 林展 陈风 罗贤懋[1] 魏慧娟[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所,北京100021

出　　处：《中国医学科学院学报》1997年第1期67-71,共5页Acta Academiae Medicinae Sinicae

摘　　要：以逆转录病毒介导，将人全长mdr-1cDNA导入人肺腺癌细胞系GLC82中，经G418和阿霉素筛选，挑选出3个阳性克隆。用mdr-1cDNA的一种特异引物在3个克隆中都扩增到1条167bp的带，表明mdr-1基因cDNA已稳定地整合在染色体基因组中。原位杂交显示转染细胞的mdr-1转录升高，免疫细胞化学发现转染细胞中P170糖蛋白呈弱阳性。MTT药敏实验表明3个克隆对阿霉素的抗药性分别是GLC细胞的6.4、7.0和8.8倍。提示在mdr-1基因cDNA转染的细胞中，mdr-1基因低表达足以大大提高细胞对药物的耐受性。Human lung cancer leads the mortality of cancers and the chemotherapy is often uneffective because of drug resistance. In order to study the role of mdr-1 gene in resistant lung cancer, the fully length mdr-1 cDNA was transferred into a sensitive lung cancer cell line GLC.The mdr-1 cDNA was constructed in a retroviral vector, pDORneo. The transfection of recombinant plasmid was carried out by lipofectin. Supernatant containing infective viruses derived from a G418 resistant clone of package cell PA317 was used to infect GLC cell which is sensitive to chemotherapeutic agents. After G418 and adriamycine selections, three P-glycoprotein positive clones were isolated and the integration of mdr-1 cDNA was demonstrated by PCR of genomic DNA. The relative resistance of 3 clones to adriamycine as and elevated by 5. 4, 6.0 respectively 7. 8 times compared with the untransfected cell and the transcription of mdr-1 gene in these transfected cells as obviously enhanced by in situ hybridization. This results suggest that the rndr-1 gene plays a role in increasing drug resistance of human lung cancer.

关 键 词：耐药性 基因转移 癌 肺肿瘤 阿霉素 MDR-1 

分 类 号：R734.205[医药卫生—肿瘤] R915[医药卫生—临床医学]