黄伞胞外多糖抗自由基作用的研究  

Antioxidation Study of Extra-polysaccharides Extracted from Pholiota adiposa(Fr.) Quel

作　　者：吉叶梅[1] 胡清秀[2] 宫春宇[1] 郭光[2] 安荣殊[3] 

机构地区：[1]中国农业科学院农业资源与农业区划研究所,北京100081 [2]河北省廊坊市香河县卫生局,河北廊坊065400 [3]中日友好医院,北京100029

出　　处：《生物技术》2007年第2期29-31,共3页Biotechnology

基　　金：农业部"农业结构调整重大技术研究专项--野生食用菌驯化培养与深加工技术研究"(编号:06-08-01A)项目资助

摘　　要：目的:以乙醇沉淀的黄伞发酵浓缩液得到的胞外多糖为研究对象,检验其对自由基的清除作用。方法:采用电子顺磁共振(EPR)波谱仪检测黄伞胞外多糖清除羟自由基OH.和超氧阴离子自由基O2-.的作用。结果:在样品浓度均为100mg/mL的条件下,利用Fenton反应体系,黄伞胞外多糖对羟自由基OH.的清除率仅为38.5%;而利用次黄嘌呤-黄嘌呤氧化酶体系,黄伞胞外多糖对超氧阴离子自由基O2-.的清除率可达到80.6%。结论:黄伞胞外多糖在体外对自由基有一定的清除作用且其对超氧阴离子自由基O2-.的清除作用明显优于对羟自由基OH.的清除作用。Objective： The extra-polysaccharides which were extracted from fermentation broth of Pholiota adiposa（Fr.） Quel with alcohol was researched in this article to check if it could scavenge free radicals.Methods： EPR was used to detect the scavenging effect of extra-polysaccharides on oxyradicals and hydroxyl.Results： Its scavenging ratio on hydroxyl was only 38.5% using the Fenton system under 100mg/mL,but when it was in oxidizing enzyme system of xanthine and xanthine oxidase the scavenging ratio of the extra- polysaccharides was up to 80.6%. Conclusion： The extra - polysaccharides extracted from fermentation broth of Pholiota adiposa （Fr.） Quel could scavenge free radicals and the extra - polysaccharides＇ effect on oxidase scavenging was better than that on hydroxyl scavenging.

关 键 词：黄伞 胞外多糖 氧自由基 羟自由基 

分 类 号：Q538[生物学—生物化学]