降钙素基因相关肽对局灶性脑缺血再灌注大鼠海马CREB和磷酸化CREB的上调作用  

CGRP upregulates the expressions of CREB and phospho-CREB protein during focal cerebral ischemia/reperfusion in rat hippocampus

作　　者：张正洪[1] 曲鹏[2] 方秀斌[2] 

机构地区：[1]郧阳医学院解剖学教研室,十堰442000 [2]中国医科大学基础医学院神经生物学教研室,沈阳110001

出　　处：《解剖学杂志》2007年第2期174-177,共4页Chinese Journal of Anatomy

基　　金：辽宁省自然科学基金(619019)

摘　　要：目的:探讨外源性降钙素基因相关肽(CGRP)对局灶性脑缺血再灌注大鼠海马CREB和磷酸化CREB(p- CREB)表达的影响。方法:制作局灶性脑缺血再灌注模型实验组并给予CGRP,应用免疫组织化学、Western印迹和图像分析方法检测大鼠海马CA1区CREB和p-CREB表达。结果:缺血再灌注组CA1区CREB表达较假手术组减少,CGRP组CA1区的CREB表达高于缺血再灌注组。缺血再灌注组CA1区p-CREB表达高于假手术组,CGRP组p-CREB表达多于缺血再灌注组。结论:CGRP上调局灶性脑缺血再灌注大鼠海马CREB和p-CREB的表达,CGRP对缺血神经元的保护作用可能是通过上调神经元内CREB和p-CREB来实现的。Objective： To investigate the effect of calcitonin gene-related peptide（CGRP） on the expression of cyclic AMP response element binding protein （CREB） and phosphorylated-CREB in rat hippocampus during focal cerebral ischemia/reperfusion（I/R）. Methods： Focal cerebral ischemia/reperfusion model was induced by occlusion of the right middle cerebral artery using the intraluminal suture method. The expressions of CREB and phospho CREB protein in the right hippocampal CA1 region were detected with immunohistochemistry and Western blotting and the positive products were analyzed by using image analysis system. Results.. The expression of CREB protein in the right hippocampal CA1 region in ischemia/reperfusion（I/R） group reduced as compared to that in the sham group, while the ex pression of CREB protein increased in CGRP group in comparison with that in I/R group. Phospho-CREB was barely detected in the right hippocampal CA1 region in the sham group and it increased more significantly in I/R group than that in the sham group. Besides, the expression of phospho-CREB in the right CA1 region in CGRP group increased further as compared to that in I/R group. Conclusion： CGRP upregulates the expressions of CREB and phospho-CREB in the ischemic neurons of the hippocampus during focal cerebral ischemia/reperfusion and CREB is probably involved in the protective mechanism of CGRP to ischemic neurons.

关 键 词：脑缺血 降钙素基因相关肽 c-AMP反应元件结合蛋白 海马 免疫组织化学 免疫印迹 

分 类 号：R743[医药卫生—神经病学与精神病学]