应用序列探针检测慢性淋巴细胞白血病基因异常  

Abnormality of genes detected by sequence-specific probes in CLL cases

作　　者：李倩[1] 杜兵[1] 杨军军[1] 刘国君[1] 季抗挺[1] 

机构地区：[1]温州医学院附属二院,浙江温州325000

出　　处：《中国卫生检验杂志》2007年第4期672-674,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨慢性淋巴细胞白血病(CLL)患者的基因异常。方法:用两种序列特异性探针D13S272(13q14.3)、ATM(11q23),一种着丝粒探针,以间期荧光原位杂交技术(FISH)选择性的对28例CLL进行检测,并和常规细胞遗传学检测结果比较。结果:常规细胞遗传学方法检出10例有克隆性染色体异常,其中5例检出+12,仅一例检出13q-,其余均为未涉及13q-、11q-的异常。两种位点特异性探针检出12例(44%)异常,del(13q14.3)9例,del(11q23)7例。12号着丝粒探针检出6例有+12,而且D13S272(13q14.3)、ATM(11q23)缺失常发生在常规检测正常的核型。3种探针异常检出率为57%(16/28)。结论:FISH技术可以检测出常规细胞遗传学方法无法检出的较小片段缺失,因此,位点特异性FISH是检测CLL患者基因异常的有效手段。Objective： To investigate the abnormalities of genes in CLL cases. Methods： By interphase fluorescence in situ hybridization（FISH） ,28 cases with CLL were detetcted by two sequence specific probes for D13S272 （13q14. 3 ）, ATM （11q23）, and one centromeric probe for 12 and compared with the results of conventional cytogenedc measuring. Results： Ten cases had elonal chromosomal aberration： 10 with elonal chromosomal abnormalities, among them, ＋ 125 eases, 13q - 1 ease, （ 1/27）, other aberrations were those not concerned with 13q- , 11q-. 12 cases （44%） aberrations were detected by two science specific probes for D13S272（ 13q14. 3） ,ATM（ 11q23）, 9 with del（ 13q14.3）, 7 with del（11q23）. Six eases for trisomy 12 were detected by one eentromerie probe for 12. And del（13q14.3） ,del（11q23） were usually detected in normal karyotype. Conclusion： By FISH, the smaller sequence deletion which can＇t be found by CC can be found. Therefore,status specified FISH is the effective method to detect genie aberrations in CLL.

关 键 词：慢性淋巴细胞白血病 位点特异性FISH 常规细胞遗传学 

分 类 号：R733.72[医药卫生—肿瘤]