干酪乳杆菌Lactobacillus casei 393在鼠消化道内定植能力及分布规律的研究  

Colonization ability and distribution of Lactobacillus casei 393 in mouse gut

作　　者：徐义刚[1] 崔丽春[2] 唐丽杰[1] 赵丽丽[1] 李一经[1] 

机构地区：[1]东北农业大学动物医学院,哈尔滨150030 [2]东北林业大学,哈尔滨150040

出　　处：《中国乳品工业》2007年第4期20-22,共3页China Dairy Industry

基　　金：国家自然科学基金项目(NO.30371074)

摘　　要：研究了干酪乳杆菌Lactobacillus casei 393在小鼠肠道中定植能力及分布规律。利用羧基荧光素二乙酸琥珀酰亚胺酯分子探针对干酪乳杆菌Lactobacillus casei 393进行标记,以每只1010mL-1的量,口服途径喂给BALB/c鼠,分别于口服后的第1,3,5,6 d取其十二指肠、空肠、回肠、结肠等肠段,通过流式细胞仪检测肠内的标记阳性菌。实验结果显示:经口服后第5 d,干酪乳杆菌Lactobacillus casei 393开始在BALB/c鼠肠道中定植,在十二指肠、空肠、回肠、结肠的定植率分别为28.70%,29.77%,15.63%,26.17%,第6 d标记的阳性菌出现明显的生长趋势,阳性检出率分别为71.77%,57.31%,65.44%,53.13%。研究表明干酪乳杆菌Lactobacillus casei 393具有良好的肠道定植能力。This study investigated the colonization potential and distribution of Lactobacillus casei 393 in mouse intestine by using 5 （and 6）- carboxyfluorescein diacetate, succinimidyl ester （CFDA-SE）. The BALB/c mice were orally dosed with approximately 10^10 CFDA-SE-labelled Lactobadllus casei 393 by orogastric administration. The positive CFDA-SE-labelled bacterium in duodenum, jejunum, ileum and colon of BALB/c mice were enumerated through the flow cytometry on days 1, 3, 5, 6 after orogastric administration. The result： showed that Lactobacillus casei 393 could colonize in the intestine of BALB/c mice on day 5, the colonization percentages were 28.70% ,29.77% ,15.63%, 26.17% in duodenum, jejunum, ileum and colon, respectively. On day 6, the labelled Lactobadllus casei 393 could grow evidently, the positive labeled bacterium percentages were 71.77%, 57.31%, 65.44%, 53.13%, respectively.

关 键 词：干酪乳杆菌 定植能力 羧基荧光素二乙酸琥珀酰亚胺酯 

分 类 号：TS201.3[轻工技术与工程—食品科学]