云锡矿工肺癌组织DAPK基因表达及其甲基化异常  

Expression and aberrant methylation of DAPK gene of lung cancer tissues in Yunnan tin miners

作　　者：华海蓉[1] 金克炜[1] 阮永华[1] 高倩[1] 

机构地区：[1]昆明医学院基础医学院病理教研室,云南昆明650031

出　　处：《中国职业医学》2007年第2期87-90,共4页China Occupational Medicine

基　　金：国家自然科学基金资助项目(30160031)

摘　　要：目的探讨云锡矿工肺癌组织DAPK基因的蛋白表达及其甲基化异常。方法收集云锡矿工肺癌组织50例及10例正常肺组织。采用免疫组化检测DAPK蛋白表达,采用原位细胞凋亡检测法检测细胞凋亡,采用甲基化特异性聚合酶链反应检测DAPK基因启动子甲基化。结果50例癌组织中,38%DAPK蛋白表达阴性,36%检测到DAPK基因启动子异常甲基化,DAPK蛋白表达与细胞凋亡有关(P<0·05),DAPK基因启动子异常甲基化与DAPK蛋白表达呈负相关(P<0·05)。结论①云锡矿工肺癌组织中DAPK基因表达减少或缺失;②云锡矿工肺癌发生发展可能与DAPK基因异常甲基化有关。Objective To investigate the protein expressions of DAPK gene and its promoter methylation status of lung cancer tissues in Yunnan tin miners. Methods 50 cases of lung cancer among Yunnan tin miners and ten normal lung tissues were obtained. DAPK protein expression was analyzed by immunohistochemistry. TUNEL method was used to detect apoptosis. DAPK methylation was assessed by methylation-specific polymerase chain reaction （MSP）. Results DAPK protein was not expressed in 38% lung tumors among Yunnan tin miners. Express of DAPK protein was correlated with apoptosis index （ P 〈 0. 05 ）. Promoter methylation of DAPK was found in 36% of lung tumors among Yunnan tin miners. In addition, promoter methylation of DAPK was significantly associated with negative immunostaining of DAPK （ P 〈 0. 05 ）. Conclusion （1） The expression of DAPK is down-regulated or loss in lung cancer tissues in Yunnan tin miners ; （2） Aberrant promoter methylation is an important mechanism in the development and progress of lung cancer in Yunnan tin miners.

关 键 词：肺癌 云锡矿工 异常甲基化 死亡相关蛋白激酶 

分 类 号：R734.2[医药卫生—肿瘤] R135.2[医药卫生—临床医学]