植酸酶基因PhyB_1的克隆及序列分析  

Cloning and Sequence Analysis of the PhyB Gene of Aspergillus niger WP1

作　　者：柯晓静[1] 郭润芳[1] 于宏伟[1] 贾英民[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001

出　　处：《华北农学报》2007年第2期14-17,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省"十一五"科技攻关项目资助(06220106D)

摘　　要：为了进一步研究多种植酸酶之间的相互作用,利用PCR技术,从黑曲霉WP1中克隆出植酸酶PhyB基因,该基因长1 560 bp,含有3段内含子,共编码460个氨基酸,与已报道的ALKO243的植酸酶Aph基因(GeneBank Acces-sion:L02420)相比较,编码的氨基酸序列同源性为99.13%,因此将其命名为PhyB1。该基因含有植酸酶保守序列“RHGXRXP”和“HD”。黑曲霉WP1植酸酶PhyB1基因序列已在国际基因库中注册,注册号为:DQ836360。The PhyB1 encoding phytase of Aspergillus niger WP1 was amplified by the polymerase Chain reaction （PCR） with primers designed according to the sequences in GeneBank. The amplified fragment was cloned and sequenced. The results showed that the coding region was 1 560 bp in size, includes 3 introns , and encoded a peptide of 460 amino acid residues . Comparison of this sequence with the Aph of the nature A. niger ALKO243 （GeneBank Accession： L02420）, shows that the nucleotide homology is 96.79 %, and the amino acid homology comes up to 99.13 %. The PhyB1 of A. niger WP1 strain in this paper its sequence has been accessed by GeneBank （Accession：DQ836360）.

关 键 词：黑曲霉 PhyB基因 PCR 序列分析 

分 类 号：Q789[生物学—分子生物学]