棉花黄萎菌间接ELISA方法的建立及其检测棉花体内带菌的应用  

Indirect ELISA for Detection of Verticillium dahliae and Its Application

作　　者：刘翠芳[1] 高广瑞 田世民[3] 蒋继志[1] 鹿秀云[4] 马平[4] 李社增[4] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北省科学技术厅,河北石家庄050021 [3]中国检验检疫科学研究院,北京100025 [4]河北省农林科学院植物保护研究所河北省农业有害生物综合防治工程技术研究中心,河北保定071000

出　　处：《华北农学报》2007年第2期145-148,共4页Acta Agriculturae Boreali-Sinica

基　　金：农业部"948"项目资助(2005-Z20)

摘　　要：利用棉花黄萎病的病原菌的菌丝蛋白作为抗原,制备了该病原菌菌丝蛋白的多克隆抗体,建立了特异性检测棉花黄萎病菌的间接ELISA方法。该方法检测灵敏度达到5 ng/mL棉花黄萎菌菌丝蛋白。应用该方法可定量检测棉花组织及其根部土壤中棉花黄萎菌的含量,同时发现病株的茎、根及根部土壤的带菌量多少与病株发病程度有一定的相关性。本研究说明,该方法可在棉花黄萎病的早期检测和预报上具有重要的应用前景。Mycelium protein of Verticillium dahliae was used for the production of polyclonal antibodys to V. dahliae, and a indirect ELISA method for specific detection of cotton verticillium wilt was established. The Mycelium protein of V. dahliae could be detected as low as 5 ng/mL at 405 nm. V. dahliae could be quantitively detected in the stem, root, leaf and soil from the diseased cotton plants through this method. Results also showed that amounts of the pathogen from the sterns, roots and the soil of the diseased plants were positive correlation to the severity of cotton verticillium wilt. It convinced that the new established Elisa method is a promising technique for early detection and forecast of cotton verticillium wilt.

关 键 词：棉花黄萎病 菌丝蛋白 多克隆抗体 ELISA 检测 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]