大鼠海马内σ受体在电针抑制电惊厥中的作用  

Effects of intrahippocampal sigma receptor on inhibition of electroconvulsive seizure by electroacupuncture (EA) in rats

作　　者：柳耀泉[1] 高焕民[1] 王少萍[1] 

机构地区：[1]青岛大学医学院第二附属医院神经科,山东青岛266042

出　　处：《中国医药导报》2007年第04X期12-14,共3页China Medical Herald

基　　金：青岛市科技局立项课题(No.2004-wszd024)。

摘　　要：目的:观察大鼠海马内σ受体在电针抑制电惊厥中的作用。方法:62只成年雄性Wistar大鼠分为6组。在大鼠电惊厥模型(通过双侧耳廓部的电极夹给予电刺激:50Hz,60mA,持续0.4s,共3次,每次间隔5min)上应用行为观察,用海马内微量注射受体激动剂或拮抗剂及脑电功率谱分析方法对惊厥及其程度进行分析;使用G6805-2电针治疗仪电针“风府”(GV16),“筋缩”(GV8),频率130Hz,强度1mA,持续10min。结果:大鼠海马内微量注射σ受体激动剂1,3-双-2-甲苯基胍(1,3,di(2-tolyl)guanidine,DTG)8.27nmol可以抑制电惊厥发作,表现为痫样放电减轻,脑电总功率和主频功率降低,并且增强电针抑制电惊厥的作用。而大鼠海马内注射σ受体拮抗剂右吗喃1.5nmol/0.5μl后则使电惊厥发作加剧,减弱了电针抑制电惊厥的作用(“针+药”组与“针+人工脑脊液”组及单纯药物组比较,P<0.01)。结论:大鼠海马内σ受体在电针抑制电惊厥中起一定的加强针效的作用。Objective： To investigate the effects of intrahippocampal sigma receptor on electroacupuncture anticonvulsion. Methods： The behavioral observations, intrahippocampal microinjections were used on the electroconvulsive seizure model （50 Hz,60 mA,0.4 s, 3 times with 5 minutes interval） in 62 male Wistar rats divided into 6 groups. EA was applied at the acupuncture points of Fengfu Point （GV. 16） and Jinshuo Point （GV. 8） by using model G6805-2 EA apparatus with continuous waveforms of 130 Hz and 1 mA. Results： Microinjection of 1,3,di （2-tolyl）guanidine （DTG,8.27nmol/ 0.5μl）,an agonist of sigma receptor, into the hippocampus reduced the epileptiform activities and enhanced the EA anticonvulsion significantly, whereas microinjection the antagonist of the sigma receptor dextrorphan 1.5nmol/0.5μl induced the provocation of epileptiform activities, and resulted in disappearance or reversal of inhibitory effects of EA on seizure. P〈0.01 respectively when compared between Drug and ACSF, Drug ＋EA, ACSF ＋EA. Conclusion： The sigma receptor in the hippocampus might play a role in electroconvulsive seizure and facilitate EA anticonvulsion effects.

关 键 词：电针 海马 电惊厥 σ受体 

分 类 号：R363.2[医药卫生—病理学]