人胰岛素酶联免疫分析法的建立  

The Establishment of an Enzyme Linked Immunosorbent Assay for Insulin

作　　者：贾娟娟[1] 刘一兵[1] 陈键[1] 许文革[1] 官国英[1] 韩世泉[1] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413

出　　处：《同位素》2007年第1期24-28,共5页Journal of Isotopes

摘　　要：采用两株抗胰岛素单克隆抗体，一株包被聚苯乙烯板、另一株标记辣根过氧化物酶，建立了测定人胰岛素的双抗体夹心酶联免疫分析法。方法学研究表明，本方法的灵敏度为1．7mIU／L，曲线范围5～160mIU／L，批内、批间变异系数分别小于11％、15％，回收率96．4％～111．1％；高浓度胰岛素血清样品系列倍比稀释后测定，测定值与稀释度呈线性相关，相关系数0．998；38例健康人血清样品测定值为3．6～10．8mIU／L。该方法与人胰岛素放射免疫分析法测定结果的相关性良好，r=0．97。Two monoclonal antibodies against insulin are applied in two-site enzyme linked immunosorbent assay （ELISA）. One of the antibodies is immobilized on the microtiter plate, the other is labelled with HRP. The sensitivity of the assay is 1.7 mIU/L and the recovery is 96.4%～111.1%. The intra-and interassay CV are below 11% and 15.0% respectively. The correlation coefficient between measured and cliluted values is 0. 998 after serial dilutions of the samples using high concentration of insulin. The value for normal samples （n=38） is 3.6～10.8 mIU/L. Compared with RIA, the correlation coefficient is acceptable （r=0.97）.

关 键 词：胰岛素 单克隆抗体 酶联免疫分析 放射免疫分析 

分 类 号：R392-33[医药卫生—免疫学] Q814.9[医药卫生—基础医学]