蜂胶提取液对大鼠急性胸膜炎的作用及其机制的研究  

STUDY ON THE EFFECT OF PROPOLIS EXTRACTS ON ACUTE PLEURISY AND ITS MECHANISM IN RATS

作　　者：胡福良[1] 李英华[2] 朱威[1] 陈民利 应华忠 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]浙江大学材料与化工学院,杭州310027 [3]浙江中医学院,杭州310053

出　　处：《营养学报》2007年第2期189-191,共3页Acta Nutrimenta Sinica

基　　金：浙江省重大科技攻关资助项目(No.2003C12021)

摘　　要：目的观察蜂胶的抗炎作用及其可能的机制。方法40只健康雄性Wistar大鼠随机分为正常对照、模型对照、阳性对照(给予醋酸强的松)、蜂胶乙醇提取液和蜂胶水提取液组。注射角叉菜胶以建立急性胸膜炎模型。测定白细胞计数及分类、血清中MDA含量、SOD活性、溶菌酶含量和GSH-PX活性及胸腔渗出液中蛋白质含量、炎症介质NO和PGE2的含量。结果蜂胶的乙醇提取液和蜂胶的水提取液可对抗胸膜炎大鼠胸水增多,抑制中性粒细胞升高,明显降低血清中的MDA含量,增加SOD活性,降低溶菌酶含量和GSH-PX活性,明显降低胸腔渗出液中的蛋白质含量,显著减少炎症介质NO和PGE2的生成,减轻炎症反应的发生程度。结论蜂胶具有良好的抗炎作用。降低NO和前列腺素间的相互作用,减少各种蛋白激酶的激活效应是其机制之一。Objective： To investigate the anti-inflammatory effect and the possible mechanism of water and ethanol extracts of propolis. Method： Forty male Wistar rats were divided into 5 groups： normal group, model group, medicine groups, two groups treated with water and ethanol extracts of propolis. The acute pleurisy model was established by injecting carrageenan. The effects of propolis on acute pleurisy was studied by counting leukocytes, measuring the content of MDA, lysozyme and activity of SOD in serum and the content of NO, protein and PGE2 in pleural effusion. Results： The propolis solutions extracted by water and ethanol presented obvious effect on inflammation. It could antagonize the purulent pleurisy, reduce the number of leukocytes and the content of MDA, lysozyme and activity of SOD in serum and the contents of NO, protein and PGE2 and decrease the inflammation. Conclusion： Propolis displays anti-inflammatory effects by decreasing the action of NO and PGE2 and preventing the activation of protein kinase.

关 键 词：蜂胶 角叉菜胶 急性胸膜炎 NO PGE2 

分 类 号：R285.5[医药卫生—中药学]