0rexin—A及其反义寡核苷酸对睡眠剥夺幼鼠空间学习记忆能力的影响  

Effects of Orexin-A and antisense oligonucleotides on spatial learning and memory in sleep-deprived young rats

作　　者：梁振江[1] 陈旭红[1] 冼志雄[1] 李兰[1] 陈静[1] 

机构地区：[1]深圳市儿童医院耳鼻喉科,深圳518026

出　　处：《中国行为医学科学》2007年第4期302-304,共3页Chinese Journal of Behavioral Medical Science

摘　　要：目的　研究Orexin-A及其反义寡核苷酸对睡眠剥夺幼鼠空间学习记忆的影响。方法　小平台水环境法建立幼年大鼠睡眠剥夺模型，同时随机分为Orexin—A注射组、Orexin-A反义寡核苷酸注射组、DMSO对照组及正常对照组，连续7d经脑室给药，给药及睡眠剥夺第3天开始进行Morris水迷宫训练及测试。结果　从水迷宫训练第2天开始，各注射组平均逃避潜伏期较正常对照组均明显延长（P〈0．01）。第4天、第5天Orexin．A注射组平均逃避潜伏期[分别为（59．06±17．34）s，（41．24±17．35）s]较DMSO对照组[分别为（44．71±16．57）s，（29．02±11．52）s]和反义寡核苷酸注射组[分别为（33．29±10．35）s，（24．21±6．36）s]显著延长（P〈0．01），反义寡核苷酸注射组平均逃避潜伏期较DMSO对照组明显缩短（P〈0．01）。各注射组穿环数与正常对照组相比均明显减少（P〈0．01）。Orexin-A注射组穿环数（4．20±1．61）较DMSO对照组（7．15±1．50）和反义寡核苷酸注射组（9．80±1．20）明显减少（P〈0．01），反义寡核苷酸注射组穿环数明显高于DMSO对照组（P〈0．05）。结论　Orexin—A参与了睡眠剥夺幼鼠空间学习记忆能力的损伤，Orexin-A　mRNA反义寡核苷酸可显著逆转这一损伤的产生。Objective To investigate Orexin-A and antisense oligonucleotides on spatial learning and memory in sleep-deprived young rats. Methods Sleep deprivation model was made by small platform method. Meanwhile, rats were divided randomly into Orexin-A injection group （ Orexin-A group）, Orexin-A mRNA antisense oligonucleotides injection group （ODN group）, DMSO injection group （DMSO group ） and normal control group（ normal group）. Drugs were given respectively by cerebralventricle everyday and lasted for 7days. At the 3rd day, rats began receiving Morris water maze training and test. The normal group didn＇t bear sleep-deprivation and drug injection. Results From the 2nd day of Morris water maze training, the average escape latency of normal group was shorter than Orexin-A group, ODN group and DMSO group（ P〈 0.01 ）. On the 4th day and the 5th day, the Orexin-A group （59.06 ± 17.34s and 41.2± 17.35s respectively） spent shorter time to locate the hidden platform than the DMSO group （44.71 ± 16.57s and 29.02 ± 11.52s respectively） and the ODN group（33.29 ± 10.35s,24.21 ± 6.36s respectively ） （ P 〈 0.01 ）. Meanwhile , the average escape latency of ODN group was shorter than the DMSO group（ P〈 O. 01 ） . Comparing with the normal group , the other three groups had less times of passing through the platform（ P〈0.01 ）. The times of passing through the platform of the Orexin-A group （4.2 ±1.61 ）was less than the ODN group （9.8 ±1.20） and the DMSO group （7.15 ± 1.50） （ P 〈 0.01 ）, and the DMSO group was less than the ODN group（ P〈 0.05）. Conclusion Orexin-A contributes to the injury of spatial learning and memory capacity in sleep-deprived young rats. Orexin-A mRNA antisense oligonucJeotides can reverse this injury partly but significantly.

关 键 词：OREXIN-A 睡眠剥夺 空间学习记忆 反义寡核苷酸 

分 类 号：R686[医药卫生—骨科学]