Hydroxylation of 3-nitrotyrosine by hydroxyl radical  

作　　者：Jun Chen Dong Wei Qun Shi Yu Fen Zhao Yan Mei Li 

机构地区：[1]Key Laboratory of Bioorganic Phosphorous Chemistry ＆ Chemical Biology （Ministry of Education）, Department of Chemistry, Tsinghua University, Beijing 100084, China

出　　处：《Chinese Chemical Letters》2007年第5期542-544,共3页中国化学快报（英文版）

基　　金：The authors would like to thank the financial support from the National Natural Science Foundation of China （No. 20320130046） ；the Specialized Research Fund for the Doctoral Program of Higher Education （SRFDP） of M0E （No. 20030003049）.

摘　　要：Hydroxylation of 3-nitrotyrosine （3-NT） and 3-NT containing peptide Gly-nitroTyr-Gly in aqueous solution by hydroxyl radical were investigated with gamma irradiation. The structures of the hydroxylated products were confirmed by electrospray ionization mass spectrometry and ^1H NMR spectrometry. The reactivity of 3-nitrotyrosine has been investigated using density functional theory （DFT） calculation.Hydroxylation of 3-nitrotyrosine （3-NT） and 3-NT containing peptide Gly-nitroTyr-Gly in aqueous solution by hydroxyl radical were investigated with gamma irradiation. The structures of the hydroxylated products were confirmed by electrospray ionization mass spectrometry and ^1H NMR spectrometry. The reactivity of 3-nitrotyrosine has been investigated using density functional theory （DFT） calculation.

关 键 词：3-NITROTYROSINE Hydroxyl radical DFT Selective hydroxylation 

分 类 号：Q517[生物学—生物化学]