白肋烟两个DH群体的构建与染色体倍性的鉴定  

Construction of two DH populations and identification of chromosome ploidy in burley tobacco

作　　者：柴利广[1] 张俊杰 林国平 王毅 徐芳森[1] 

机构地区：[1]华中农业大学农业部亚热带农业资源与环境重点开放实验室,武汉430070 [2]中国烟草白肋烟试验站,武汉430030

出　　处：《中国烟草学报》2007年第2期33-37,共5页Acta Tabacaria Sinica

基　　金：国家烟草专卖局重点资助项目(110200202004)。

摘　　要：分别采用气孔保卫细胞叶绿体计数和流式细胞仪两种方法,鉴定了白肋烟烟碱含量和抗黑胫病两个杂交组合的杂种F1代经花药培养获得的再生植株的染色体倍性,结果发现叶绿体计数法可以简单经济地检测烟草花药培养再生植株的染色体倍性,但由于受混倍体植株的影响,鉴定结果的准确性较低。流式细胞仪则更加简便快速而且准确地鉴定出各种染色体倍性的再生植株,鉴定结果与开花结实情况完全一致。在此基础上构建了可用于白肋烟烟碱合成代谢和抗黑胫病遗传研究及基因定位的两个双单倍体(DH)遗传群体。Two methods, i.e. chloroplast count in stoma guard cell in leaves and flow-cytometry, were used to identify chromosome ploidy of regenerated plants obtained by anther culture from F1 hybrids of two hybridization combinations respectively related to nicotine content and black shank resistance in burley tobacco. Results showed that the method of chloroplast count was simple and economical in identifying chromosome ploidy. However, its veracity was limited due to the existence of mixoploid in the regenerated plants. Comparatively, flow-cytometry was a more efficient and accurate method. Two doubled haploid （DH） populations were constructed based on anther culture and chromosome ploidy identification and may be used to study the genetic base and the gene mapping for nicotine anabolism and black shank resistance, respectively.

关 键 词：白肋烟 DH群体 染色体倍性 气孔保卫细胞叶绿体 流式细胞仪 

分 类 号：S572[农业科学—烟草工业]