多西紫杉醇节律化疗联合地塞米松抗小鼠肺癌血管生成的实验研究  

Experimental studies of metronomic chemotherapy with docetaxel followed by dexamethasone on tumor growth and angiogenesis on mouse Lewi＇s lung carcinoma model

作　　者：蔡政[1] 胡秀峰[2] 周芳[1] 韩颖[1] Soumitra sudip bhuyan 李醒亚[1] 

机构地区：[1]郑州大学第一附属医院肿瘤科,郑州450052 [2]河南省肿瘤医院

出　　处：《中原医刊》2007年第8期5-7,共3页Central Plains Medical Journal

基　　金：国家自然科学基金（30672423）

摘　　要：目的探讨多西紫杉醇节律化疗及地塞米松对C57BL／6小鼠Lewis肺癌（LLC）肿瘤生长和血管生成的影响。方法C57BL／6小鼠皮下接种LLC细胞，随机分组后分别给予相应治疗。隔天测量小鼠体重及肿瘤体积。小鼠处死后称瘤重，应用免疫组化检测小鼠肿瘤组织中CD34和HIF-1α蛋白表达。结果对照组皮下瘤重明显大于节律组、地塞米松组和联合组。对照组微血管密度（MVD）明显高于三个实验组（P〈0．05），三个实验组HIF-1α蛋白表达较对照组显著下降（P〈0．05）。结论多西紫杉醇节律化疗和地塞米松可明显抑制LLC的生长及血管生成，其可能的机制之一是通过下调小鼠肿瘤组织中HIF—1α的表达而间接实现抗肿瘤血管生成作用。Objective To discuss the effects of metronomic chemotherapy with docetaxel followed by dexamethasone on tumor growth and angiogenesis on C57BL/6 mouse Lewi＇s lung carcinoma（LLC） model. Methods LLC cells were injected subcutaneously into C57BL/6 mice. According therapy was given to randomized groups. Mice weight and tumor diameter were measured every other day. Then tumors were weighed after killing the mice. CD34 and HIF - 1 a protein expression were detected in tumor tissues of mice by immunohistochemistry. Results The tumor weight of control group was heavier than that of metronomic group, dexamethasonegroup and combination group. The average microvessel density of control group was remarkblely higher than that of other three experiment group（ P 〈 0. 05 ）. Conclusion Metronomic chemotherapy with docetaxel fol-lowed by dexamethasone significantly inhibits tumor growth and angiogenesis in mouse LLC model. One of potential mechanisms is to implement indirectly anti - tumor angigenesis by downreguahing HIF - a protein expression of mouse tumor tossues.

关 键 词：节律化疗 地塞米松 血管生成 缺氧诱导因子-1α 

分 类 号：R734[医药卫生—肿瘤]