环氧化酶2在肝衰竭模型肝损伤中的表达  

Expression of cyclooxygenase - 2 in lipopolysaccharide mediated fulminate hepatic failure

作　　者：施柏年[1] 刘江[1] 何建方[1] 戴利成[1] 周建方[1] 赵卫锋[2] 甘建和[2] 

机构地区：[1]浙江省湖州市中心医院感染内科,湖州313000 [2]苏州大学附属第一医院感染内科

出　　处：《中原医刊》2007年第9期12-15,共4页Central Plains Medical Journal

摘　　要：目的探讨环氧化酶2（COX2）在不同剂量内毒素诱导的鼠肝衰竭肝损伤的表达。方法分为正常对照组、肝衰竭组、预处理组。采用不同剂量的脂多糖（LPS）（2、4、8mg／kg，A、B、C亚组）尾静脉注射诱导复制肝衰竭模型。检测同一时点血浆的丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、白细胞介素-10（IL-10）、肿瘤坏死因子α（TNF-α）、细胞粘附因子-1（ICAM-1），评价肝损伤情况；分离肝脏Kupffer细胞后用半定量RT—PCR分析COX2 mRNA的表达。用选择性COX2抑制剂预保护后，检测肝衰竭模型的上述指标的变化。结果ALT、AST、IL-10、TNF—α、ICAM—1、COX2 mRNA／β-aetin随LPS诱导剂量增加而增高；预处理组的ALT、AST、TNF—α、ICAM-1、COX2 mRINA／β-actin较肝衰竭组明显降低，差异有统计学意义。结论COX2参与了LPS诱导的肝衰竭模型肝损伤过程，与LPS存在量效关系；COX2可以作为肝衰竭模型肝损伤中干预的一个靶。Objective To study expression of cyclooxygenase -2 in lipopolysaccharide mediated fulminate hepatic failure model. Methods The animal model of fulminate hepatic failure was induced by different doses LPS（2,4,8 mg/kg, A, B, C subgroup）via tail vein respectively. To evaluate the hepatic injury of the model, plasma transaminases （ALT,AST）,IL- 10,TNF-α,ICAM- 1 were detected at different time （2,4,6,8 h） by ELISA kit after injection;expression of COX2 mRNA in kuffer cells separated from liver was detected by semiquantitative RT- PCR and its corresponding software. Pretreated by selective cyclooxygenase -2 inhibitor, changes of above -mentioned index were analyzed in fulminate hepatic failure model and the pretreated model. Results The levels of COX2 mRNA,ALT,AST,IL - 10,TNF -α,ICAM - 1 increased gradually with the increase close of LPS in A, B, C subgroup. Compared with fulminate hepatic failure model, the levels of COX2 mRNA,ALT,AST,TNF- α,ICAM- 1 in pretreated model degraded significantly except IL- 10. Conclusion COX2 is involved in fulminate hepatic failure model. It has dose - dependent relationship with LPS. Selective cyclooxygenase -2 inhibitor can amend LPS mediated liver injury. Cyclooxygenase -2 could be a target of liver injury in animal fulminate hepatic failure model.

关 键 词：环氧化酶2 内毒素 肝衰竭 选择性COX2抑制剂 

分 类 号：R575[医药卫生—消化系统] R-332[医药卫生—内科学]