鼻咽癌患者血清VCAM-1表达及其与IL-1β、IL-4等细胞因子的关系  

Expresion of serum VCAM-1 and their relationships with IL-1β,IL-4,in patients with nasopharyngeal carcinoma

作　　者：王东明[1] 罗志飞[1] 陈明净[1] 陆海霞[1] 陈珍[2] 李群[1] 

机构地区：[1]海南医学院病理学教研室,海南海口571101 [2]海南医学院附属医院耳鼻咽喉科,海南海口570102

出　　处：《海南医学院学报》2007年第1期11-16,共6页Journal of Hainan Medical University

基　　金：海南省自然科学基金(30316);海南医学院苗圃基金(200204)

摘　　要：目的:观察鼻咽癌患者血清血管内皮细胞粘附因子-1(VCAM-1)和IL-1β、IL-2、IL-4、IL-10表达,了解鼻咽癌患者血清VCAM-1表达规律及其与诱导因子IL-1β、IL-4的关系;获得有关鼻咽癌免疫环境的信息。方法:用ELISA方法检测34例鼻咽癌患者和13例慢性鼻咽炎患者血清标本中VCAM-1、IL-1β、IL-2、IL-4和IL-10浓度。结果:鼻咽癌患者血清VCAM-1水平明显高于慢性鼻咽炎组(P=0.045);临床晚期明显低于早期(P=0.045),且随肿瘤体积增大而降低(P=0.002);鼻咽癌患者血清VCAM-1水平与IL-1β、IL-4无明显相关(P=0.101和0.116);血清IL-1β、IL-4和IL-10水平在鼻咽癌和慢性鼻咽炎组间无显著性差异;鼻咽癌组血清IL-2水平明显高于慢性鼻咽炎组(P=0.037),且随肿瘤体积增大而升高(P=0.002),但当出现淋巴结转移时则明显降低(P=0.018)。结论:鼻咽癌患者血清VCAM-1升高是一临床早期事件;鼻咽癌患者血清VCAM-1变化与IL-1β、IL-4无明显关系;IL-4和IL-10指标在鼻咽癌和鼻咽炎组间无明显变化,显示Th2主导的体液免疫在鼻咽癌形成和发展过程中无明显激活迹象;IL-2指标的变化提示鼻咽癌细胞可能有较强的刺激细胞免疫作用。Objective： To observe the expressions of serum VCAM-1, IL-1β, IL-2, IL-4, IL-10 in the patients with nasopharyngeal carcinoma （NPC） to get information about the rule of serum VCAM-1 expression and whether the relation between VCAM-1 and it＇s inducible factors IL-1β, IL-4 exist;the immune environment of NPC. Methods： ELISA was used to test the levels of VCAM-1, IL- 1β, IL-2, IL-4 and IL-10 in serum from 33 patients with NPC and 7 patients with chronic nasopharyngitis （cNPi）. Results： The level of serum VCAM-1 in patients with NPC was higher than that of in patients with cNPi （P=0. 045）. The level of serum VCAM-1 at early clinical stage of NPC was higher than that of at late clinical stage （P=0. 045）. Small tumor size （T1＋2） means a higher level of serum VCAM-1 than big tumor size （T3＋4） does （P=0. 002）. No significant correlation exists between serum VCAM-land IL-1β,IL-4 （P=0. 101, 0. 116）. The levels of serum IL-1β, IL-4, IL-10 were no sig nifieant differences between NPC and cNPi. The level of serum IL-2 in NPC was higher than that in eNPi （P=0. 037）, and also higher in T3＋4 group than that in T1＋2 group （P=0. 002）. But it was lower in NPC with node metastasis group than that without node metastasis （P=0. 018）. Conclusion： The over expression of serum VCAM-1 in NPC is an early event. The changes of serum VCAM-1 level is independent of its inducible factors IL-1β, IL-4. The humoral immunity in NPC is not be activated reflected by unchanged IL-4, IL-10 in NPC and cNPi. The tumor cells in NPC tissue might have a stimulating effect on NPC cell-mediated immunity.

关 键 词：鼻咽 癌 VCM-1 IL-1Β IL-4 IL-2 IL-10 

分 类 号：R739.63[医药卫生—肿瘤]