肉苁蓉细胞悬浮培养的代谢动力学研究  

Metabolic Dynamic of Suspension Culture of Cistanche deserticola

作　　者：李春[1] 李娟[1] 沈慧慧[1] 易丽娟[1] 刘慧英[1] 

机构地区：[1]石河子大学生物化工实验室

出　　处：《天然产物研究与开发》2007年第2期193-197,共5页Natural Product Research and Development

基　　金：石河子大学工科振兴计划专项基金(GKZX2004-02)

摘　　要：通过研究肉苁蓉细胞悬浮培养过程中碳源、氮源、磷源的消耗,pH、电导率的变化,以及细胞的生长、胞内外蛋白质含量和次生代谢产物苯乙醇苷、总黄酮和多糖合成的情况,掌握了细胞生长、营养消耗与次生代谢产物积累的基本规律,为建立结构化动力学模型奠定了基础。In the suspension culture of Cistanche deserticola ,the consumption of nutrition substrate including carbon, nitrogen and phosphate source were investigated. The changes of pH and conductance rate, cell growth, soluble proteins and secondary products including phenylethanoid glycosides, total flavones and polysaeeharides were analyzed. The regulations among growth of cell, the consumption of nutrition and the accumulation of secondary metabolites were grasped. All these could be a basis for establishing the structural dynamic model and optimizing the culture conditions.

关 键 词：悬浮培养 代谢动力学 苯乙醇苷 肉苁蓉 

分 类 号：Q813[生物学—生物工程]