用于检测氯霉素的免疫原和包被原的制备  

DEVELOPMENT OF THE IMMUNOGEN AND COATINGEN FOR RAPID DETECTION OF CHLORAMPHENICOL

作　　者：杨小姣[1] 张建新[1] 高志贤[2] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]军事医学科学院卫生学环境医学研究所

出　　处：《解放军预防医学杂志》2007年第2期87-90,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：国家科技支撑计划项目课题(No.2006BAK02A09);天津市科技攻关项目课题(No.06YFGZSH02200);广州市科技攻关项目课题(No.2006Z2-E0111)

摘　　要：目的制备用于氯霉素快速免疫分析的免疫原和包被原,为氯霉素胶体金免疫层析试纸条的制备提供基础。方法分别采用活性酯法和重氮化法,将半抗原氯霉素与载体牛血清白蛋白(BSA)和卵清白蛋白(OVA)偶联,用紫外光谱、红外光谱鉴定偶联结果并计算结合比。结果上述两种方法与BSA,OVA的偶联物摩尔消光系数分别为164523、49402;372723、44601,显著大于半抗原和载体蛋白。红外光谱分析进一步证实偶联成功。结论全抗原偶联成功,活性酯法和重氮化法使氯霉素半抗原与BSA,OVA的偶联比值分别为12,2;30,1。Objective To synthesize the immunogen and coatingen of chloramphenicol, so as to provide the basis for development of immunochromatographic colloidal gold test strip for rapid detection of chloramphenicol. Methods The active ester reaction and diazotization reaction were employed respectively to synthesize the hapten of chloramphenicol with crrier of bovine serum albumin （BSA）, and oval albumin （OVA）, and identified with UV - scanning and Infrared spectrometry（IRS）. Results The molar extinction coefficients of hapten with BSA, OVA by the above two methods were 164523,49402; 372723,44601 respectively, and were obviously greater than those of hapten and cartier protein alone. Conclusion The antigen of chloramphenicol was successfully synthesized. The coupling ratio values of hapten to BSA and OVA by active ester reaction and diazotizition reaction were 12, 2 and 30, 1 respectively.

关 键 词：氯霉素 免疫原 包被原 抗原 

分 类 号：R967[医药卫生—药理学]