白花丹参根制剂对胃癌细胞增殖和凋亡的影响  

Effects of SaMa miltiorrhiza Bge.f.alba root preparation on the proliferation and apoptosis of gastric cancer cells

作　　者：焦鹏[1] 常起[2] 杨明峰[1] 张凤[1] 赵晓民[1] 夏作理[3] 

机构地区：[1]泰山医学院生命科学研究所,山东省泰安市271000 [2]泰山医学院附属医院人事科,山东省泰安市271000 [3]泰山医学院微循环重点实验室,山东省泰安市271000

出　　处：《世界华人消化杂志》2007年第8期820-823,共4页World Chinese Journal of Digestology

摘　　要：目的:研究白花丹参根制剂对体外培养的人胃癌细胞BGC823增殖和凋亡的影响．方法:用不同浓度白花丹参根制剂处理胃癌细胞BGC823后,噻唑蓝(MTT)检测细胞的相对存活率,流式细胞仪Annexin V／PI双染法检测BGC823细胞凋亡,激光扫描共聚焦显微镜检测细胞形态．对结果进行统计分析,均数比较用t检验．结果:不同浓度白花丹参根制剂作用于BGC823细胞后,细胞的存活率明显下降且呈剂量依赖性(0．4 g／L:91．7％±10．6％;0．8 g／L:66．8％±5．1％;1 g／L:57．5％±9．6％;1．5 g／L:32．6％±7.3％;2 g／L:29．4％±9．4％)．镜下可见凋亡细胞典型形态,流式细胞术分析表明经过白花丹参根制剂处理后早期凋亡细胞数和晚期凋亡细胞数与对照组相比均显著增加(2．950％±1．575％vs4．105％±2．393％．P<0．05;3．848％±2．264％vs 21．465％±6．474％,P<0．05)．结论:白花丹参根制剂能有效地抑制胃癌细胞增生和诱导细胞凋亡．AIM： To investigate the effects of Salvia miltiorrhiza Bge.f.alba root preparation （SMRP） on the proliferation and apoptosis of human gastric cancer cell line BGC823 cultured in vitro. METHODS： Human gastric cancer cells BGC823 cultured in vitro were treated with different concentrations of SMRP, and then the relative survival rate of the cells in each group was examined by MTT method. Flow cytometry was used to detect the apoptosis of BGC823 cells and laser scanning confocal microscopy （LSCM） was used to demonstrate cell morphology. Statistical analysis was performed and t test was used to compare the mean value between the different groups. RESULTS： After treatment with different concentrations of SMRP, as measured by MTT assay, cell viability decreased in a dose-dependent manner （0.4 g/L： 91.7% ± 10.6%; 0.8 g/L： 66.8% ± 5.1%; 1 g/L： 57.5% ± 9.6%; 1.5 g/L： 32.6% ± 7.3%; 2 g/L： 29.4% ± 9.4%）. BGC823 cells cultured with SMRP showed the typical morphologic characteristics of apoptosis. Flow cytometry showed that the apoptotic rates in the early and late stage after SMRP treatment were significantly higher than those in the controls （4.105% ± 2.393% vs 2.950% ± 1.575%, P 〈 0.05; 21.465% ± 6.474% vs 3.848% ± 2.264%, P 〈 0.05）. CONCLUSION： SMRP can inhibit the proliferation and induce the apoptosis of human gastric cancer cells.

关 键 词：白花丹参根制剂 胃癌 细胞凋亡 细胞增殖 

分 类 号：R735.2[医药卫生—肿瘤]