白念珠菌总蛋白质组二维电泳条件的建立和优化  

Establishment and optimization of two-dimensional gel electrophoresis condition for Candida albicans proteome analysis

作　　者：阎澜[1] 张军东[1] 曹永兵[1] 高平挥[1] 姜远英[1] 

机构地区：[1]第二军医大学药学院药理学教研室,上海200433

出　　处：《第二军医大学学报》2007年第4期438-441,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30672626);上海市基础研究重点项目(03JC14006;04JC14003)~~

摘　　要：目的:建立并优化白念珠菌总蛋白质组分析的二维电泳条件。方法:以白念珠菌国际标准菌株SC5314为材料,制备其总蛋白质组样品。以真菌细胞破碎方法、裂解液成分、上样量、固相pH梯度胶条的选择为侧重点,通过不同条件下电泳图谱的比较建立最佳二维电泳技术条件。结果:成功建立白念珠菌总蛋白质制备方法,优化了白念珠菌蛋白质组分析的二维凝胶电泳技术,获得重复性和分辨率都较理想的白念珠菌总蛋白质二维电泳图谱。结论:优化后的二维电泳条件符合白念珠菌的特点,为白念珠菌蛋白质组分析奠定基础。Objective：To develop and optimize a two-dimensional（2-DE） gel electrophoresis condition for Candida albicans proteome analysis. Methods： Total cellular protein of Candida albicans strain SC5314 was extracted and .subjected to 2-DE under different conditions. The optimal conditions for sample preparation and 2-DE were screened by comparing the following parameters： cell broken methods, contents of lysis buffer, on-gel protein contents, and linear or non-linear immobilized pH gradient gels. Results： We successfully established the method for Candida albicuans protein sample preparation and optimized 2-DE condition for Candida albicans proteome analysis. A satisfactory 2-DE map with good repetition and resolution was obtained. Conclusion： The optimized condition of 2-DE in this article is suitable for Candida albicans strain, which paves a way for analysis of global protein profile in Candida albicans.

关 键 词：白念珠菌 蛋白质组 电泳 凝胶 双向 

分 类 号：R739.4[医药卫生—肿瘤]