肺鳞癌mtDNA D-环序列微卫星不稳的研究  

MSI mutation of mitochondrial DNA in lung squamous carcinoma

作　　者：刘丽红[1] 胡义德[1] 钱海洪[1] 钱频[1] 张国强[1] 

机构地区：[1]第三军医大学新桥医院全军肿瘤诊治中心

出　　处：《第三军医大学学报》2007年第10期889-891,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30080030)~~

摘　　要：目的分析肺鳞癌患者癌组织线粒体DNA(mitochondrial DNA,mtDNA)非编码D-环区序列的微卫星不稳(mitochondrial microsatellite instability,mtMSI)现象并探讨其意义。方法应用改良一步法制备15例肺鳞癌患者癌组织mtDNA,PCR产物直接测序,对比分析D-环区序列mtMSI情况。结果15例肺鳞癌患者癌组织mtDNAD-环区中,全部出现了mtMSI(100%),在3个不同位点上共发现30个mtMSI。结论肺鳞癌患者mtMSI发生率高,D环区碱基序列的mt-MSI可能与肿瘤的发生发展有关。Objective To detect the microsatellite instability （MSI） of mitochondrial DNA （mtDNA） in lung squamous carcinoma and to investigate its significance in carcinogenesis. Methods Carcinoma tissues were obtained from 15 cases of lung squamous carcinoma patients and mtDNA was extracted by one-step method. D-loop fragments were amplified by PCR. mtMSI were determined by DNA sequencing. Results The mtMSI as detected in all of 15 lung squamous carcinoma patients. Thirty mtMSIs were found in 3 different nucleotide regions. Conclusion These results indicate that the frequency of mtMSI in lung squamous carcinoma patients is very high and mtMSI in D-Loop might play an important role in lung carcinogenesis.

关 键 词：肺鳞癌 线粒体DNA D-环区 微卫星不稳 

分 类 号：R394.3[医药卫生—医学遗传学] R730.261[医药卫生—基础医学]