大鼠前额叶皮层锥体细胞中Orexin A对谷氨酸介导电流的调节效应  

Regulation of glutamate current by orexin A on pyramidal neurons in rat prefrontal cortex

作　　者：张春青[1] 夏建霞[2] 陈鹏慧[1] 胡志安[2] 

机构地区：[1]第三军医大学基础医学部神经生物学教研室,重庆400038 [2]第三军医大学基础医学部生理学教研室,重庆市神经科学研究所重庆400038

出　　处：《第三军医大学学报》2007年第10期922-924,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30370465)~~

摘　　要：目的研究前额叶皮层（prefrontal cortex,PFC）锥体细胞中觉醒肽orexinA对谷氨酸受体电流的调节效应。方法采用酶加机械分离法分离10-14 d鼠龄的Wistar大鼠PFC Ⅴ、Ⅵ层锥体细胞,利用膜片钳全细胞记录技术记录orexin A对谷氨酸诱发的跨膜电流的影响。结果Orexin A和谷氨酸均能以剂量依赖性的方式使PFC锥体神经元产生内向电流,将最大有效浓度1mmol/L的谷氨酸诱发电流的幅值作为对照组（100%）,灌流1μmol/L orexin A孵育4-10s后,再次给予1mmol/L谷氨酸,诱发电流的幅值明显增大（46.59±15.19）%（n=8,P〈0.01）。结论OrexinA对PFC锥体细胞谷氨酸受体具有显著的正向调控作用。Objective To investigate the modulatory effect of orexin A on glutamate receptor-mediated current in the freshly isolated pyramidal neurons from the rat prefrontal cortex （PFC）. Methods Deep layet （ Ⅴ - Ⅵ） prefrontal cortical pyramidal neurons from postnatal 10 to 14 day-old Wistar rats were acutely dissocit ated by a combination of mechanical and enzymatic method. Subsequently, the effect of orexin A on the curremt induced by glutamate was studied by the technique of whole cell patch clamp. Results Both orexin A and glutamate dose-dependently evoked the inward transmembrane current. The current was evoked by 1 mmol/L glutamate as a control group（ 100% ）. After treatment with 1 mmol/L orexin A for 4 - 10 s, 1 mmol/L glutamate induced-current was increased by （46.59 ± 15.19） % （ n = 8, P 〈 0.01 ）. Conclusion Glutamate receptor mediated current is markedly potentiated by orexin A in the freshly isolated pyramidal neurons, which suggest., that a synergistic action between orexin and glutamate systems exists in PFC.

关 键 词：OREXIN 谷氨酸 觉醒 

分 类 号：R338.25[医药卫生—人体生理学] R338.8[医药卫生—基础医学]