p53蛋白在低剂量电离辐射诱导EL-4细胞适应性反应中的变化  

Change of p53 protein in adaptable reaction of EL-4 cells induced by low dose ionizing radiation

作　　者：程光惠[1] 赵红光[1] 李艳博[1] 郭伟[1] 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,长春130021

出　　处：《肿瘤》2007年第4期269-271,共3页Tumor

摘　　要：目的:研究低剂量电离辐射诱导EL-4细胞适应性反应中p53 mRNA及蛋白的表达,为探讨低剂量电离辐射诱导细胞适应性反应的DNA损伤修复机制提供实验依据。方法:将EL-4细胞分为空白对照组,单纯照射组(其照射剂量分别为1 Gy、2 Gy和3 Gy),以及诱导适应性反应照射组(其照射剂量分别为75 mGy+1 Gy、75 mGy+2 Gy、75 mGy+3 Gy)。应用流式细胞仪和RT-PCR方法分别检测p53蛋白及mRNA水平表达。结果:单纯照射组p53蛋白水平较对照组显著升高(P<0.01),预先给予75 mGy照射诱导的适应性反应组其p53蛋白水平较单纯照射组则显著降低(P<0.01)。p53 mRNA水平表现出与p53蛋白表达相同的变化。结论:p53蛋白在诱导适应性反应照射组中低表达,说明其在低剂量电离辐射诱导适应性反应中可能起重要作用。Objective：To investigate the expression of p53 protein in the adaptable reaction of EL-4 cells induced by low dose ionizing radiation （ LDR）, which may provide the experimental clues for studying the repair mechanism of DNA damage in the adaptable reaction of EL-4 cells induced by LDR. Methods： EL-4 cells were divided into control group, radiation groups （ the radiation dosage was 1, 2, and 3 Gy）, and adaptable radiation groups （75 mGy ＋1 Gy, 75 mGy ＋2 Gy, and75 mGy ＋3 Gy）. The p53 mRNA and protein expression in EL-4 cells was measured by flow cytometry and RT-PCR methods, respectively. Results：The expression levels of p53 protein in the three adaptive radiation groups were significantly higher than the normal control group （P 〈0.01 ）. Adaptive radiation at 75 mGy induced adaptable reaction of EL-4 cells and decreased the expression level of p53 protein compared with control group （ P 〈0.01 ）. The expression level of p53 mRNA showed the same change as its protein level in EL-4 cells. Conclusion：The expression level of p53 protein is decreased in the adaptive radiation group, which suggests that p53 might play important roles in the adaptable reaction of EL-4 cells induced by LDR.

关 键 词：淋巴瘤 辐射 电离 肿瘤抑制蛋白质P53 EL-4细胞 

分 类 号：R730.55[医药卫生—肿瘤]