P63蛋白在人胚涎腺发育中的表达及意义  

Function of P63 on the Development of Salivary Glands

作　　者：李怡宁[1] 何志秀[2] 刘来奎[3] 何华伟[2] 

机构地区：[1]浙江大学口腔医院病理科,浙江杭州310031 [2]四川大学华西口腔医院病理科,四川成都610041 [3]南京医科大学口腔医学院口腔解剖生理学教研室,江苏南京210029

出　　处：《华西口腔医学杂志》2007年第2期111-114,共4页West China Journal of Stomatology

摘　　要：目的探讨P63蛋白在涎腺上皮干细胞发育中的作用及干细胞存在的可能位置。方法收集不同发育阶段的合法流产人胚24例、正常涎腺组织标本10例,对切片行常规HE染色和免疫组织化学SP法染色。结果组织切片见涎腺发育从早期上皮芽增生分支形成树枝状上皮条索,然后形成管腔,最终分支末端细胞增生膨大分化为导管、肌上皮和腺泡细胞。P63的表达从胚胎早期上皮芽中的广泛阳性表达到阳性细胞逐渐减少,呈现区域化散在分布特征,正常成人涎腺中阳性表达细胞仅少量存在于闰管、分泌管、排泄管管壁基底侧。结论P63在维系涎腺上皮性干细胞的生存和分化状态中可能具有重要作用,正常成人涎腺上皮性干细胞仅少量存在于导管管壁基底侧。Objective To study the expression of 1〉63 in human salivary gland development and the existing of salivary gland stem cells. Methods 24 embryonic salivary gland samples in different stage, 10 normal salivary gland samples were collected. HE-stained and immunochemistry stain were used. Results It could be seen on the HE-stained sections that the epithelial buds proliferated to form the epithelial branches and duct systems, finally the terminal cells differentiated into ductal, myoepithelial and acinous. During the development of salivary gland, the expression of 1〉63 was gradually reduced. In normal adult salivary gland samples, the positive cell interspersed in the basal layer of intercalated duct, secretory duct and excretory duct. Conclusion 1〉63 plays an important role in human salivary gland development. The result of our experiment shows the distributive characteristic of salivary gland stem cells, which exist abroad in bud stage, but decrease and only exist in the basal layer of ducts in normal adult salivary gland.

关 键 词：P63 涎腺发育 干细胞 

分 类 号：R78[医药卫生—口腔医学]