维甲酸诱导牙周韧带细胞分化的研究  

Effects of Retinoic Acid on Differentiation of Periodontal Ligament Cells

作　　者：王燕[1] 杨丕山[1] 樊明文[2] 

机构地区：[1]山东大学口腔医院牙体牙髓科,山东济南250012 [2]口腔生物医学工程教育部重点实验室武汉大学,湖北武汉430079

出　　处：《华西口腔医学杂志》2007年第2期198-201,共4页West China Journal of Stomatology

基　　金：山东省医药卫生科技发展资助项目(21350024060610);山东省中青年科学家科研奖励基金资助项目(02BS096)

摘　　要：目的研究并比较维甲酸对牙周韧带细胞和牙龈成纤维细胞向成骨样细胞分化潜能的影响。方法体外培养牙周韧带细胞与牙龈成纤维细胞,通过生化法、原位杂交及RT-PCR分别检测维甲酸作用前后碱性磷酸酶(ALP)活性的变化及其mRNA的表达情况。结果正常牙周韧带细胞与牙龈成纤维细胞的ALP活性有明显差别,前者高于后者;原位杂交及RT-PCR均检测到牙周韧带细胞有mRNA表达而牙龈成纤维细胞无表达。维甲酸作用后牙周韧带细胞ALP活性明显升高,mRNA表达信号相应增强,且在5×10-6 mol/L组效果最明显;牙龈成纤维细胞ALP活性亦有升高,在mRNA水平仅RT-PCR检测到5×10-6mol/L组有较弱的表达,其他组及原位杂交均未见表达。结论牙周韧带细胞和牙龈成纤维细胞在向成骨方向的分化潜能上存在差异。Objective To investigate the effect of retinoic acid on differentiation of periodontal ligament ceils （PDLCs） and gingival fibroblasts （GFs）. Methods The periodontal ligament ceils and gingival fibroblasts were cultured, challenged with different concentrations of retinoic acid in medium and detected for the alkaline phosphatase （ALP） activity and its mRNA by biochemical technique, in situ hybridization and RT-PCR. Results ALP activity in normal PDLCs was higher than that in GFs. The mRNA signals were positive in PDLCs, and negative in GFs. After treated with different concentrations of retinoic acid, ALP activity of PDLCs was increased than that of the control, and its mRNA signals were enhanced, especially in 5×10^-6 mol/L. The treated GFs showed a slight increase of ALP activity and a weak band of mRNA signals only in 5×10^-6 mol/L concentration. Conclusion There were differences between PDLCs and GFs in differentiating into osteoblast-like ceils.

关 键 词：牙周韧带细胞 牙龈成纤维细胞 碱性磷酸酶 维甲酸 

分 类 号：R78[医药卫生—口腔医学]