AdTERT-TRAIL对腺样囊性癌细胞株SACC-83靶向转基因作用的研究  

Targeting Transgene Effect of AdTERT-TRAIL on Salivary Adenoid Cystic Carcinoma Cell Line SACC-83

作　　者：苏涛[1] 孙宏晨[2] 郑长玉 张红[1] 卢东民[1] 

机构地区：[1]湖州师范学院医学院口腔系,浙江湖州313000 [2]吉林大学口腔医院病理科,吉林长春130041 [3]美国国立卫生研究院牙颅面研究中心

出　　处：《华西口腔医学杂志》2007年第2期202-205,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30672338);高等学校博士学科点专项科研基金资助项目(20030183068)

摘　　要：目的探讨人端粒酶逆转录酶(hTERT)启动子调控TRAIL基因在端粒酶阳性的涎腺肿瘤细胞SACC-83中靶向表达的作用。方法通过同源重组构建腺病毒载体AdTERT-TRAIL,并转染SACC-83细胞和端粒酶阴性的HEL细胞,通过RT-PCR检测TRAIL基因表达,MTT法检测TRAIL基因表达对细胞增殖的影响,流式细胞仪检测细胞凋亡率。结果转染AdTERT-TRAIL后,TRAIL基因在SACC-83细胞中明显表达,对该肿瘤细胞的增殖有明显的抑制作用,而且诱导细胞凋亡比率增加;而在HEL细胞中未能诱导TRAIL基因表达,对该细胞的增殖没有影响,也没有诱导明显的细胞凋亡。结论成功构建腺病毒载体AdTERT-TRAIL,对涎腺肿瘤细胞具有靶向转基因作用。Objective To investigate the targeting expression of TRAIL gene driven by human telomerase reverse transcriptase （hTERT） promoter in SACC-83 cell with telomerase activity. Methods Adenovirus vector AdTERT- TRAIL was constructed by homologus recombination. After transfecting AdTERT-TRAIL into SACC-83 cell and HEL cell, its effect on these ceils in vitro was investigated using RT-PCR technique, MTT method and flow cytometry. Results After transfection of AdTERT-TRAIL, expression of extrinsic TRAIL gene driven was detected in SACC-83, the proliferation of SACC-83 cell showed significant inhibitory effect （the relative cell viability was 49.70%） and its apoptotic rate was promoted （30.49%）, whereas no TRAIL gene was detected in HEL cell, also no inhibitory effect was observed in HEL cell and its apoptotic rate showed little change. Conclusion Adenovirus vector AdTERT- TRAIL was successfully constructed, which can be used to induce expression of TRAIL gene in SACC-83 cell with targeting effect.

关 键 词：人端粒酶逆转录酶启动子 腺病毒 涎腺肿瘤 

分 类 号：R739.87[医药卫生—肿瘤]