结缔组织生长因子对视网膜色素上皮细胞增生和黏附的调节作用  

Modulation of adhesion and proliferation of retinal pigment epithelium cells by recombinant human connective tissue growth factor

作　　者：郭长梅[1] 惠延年[1] 王雨生[1] 田艳明[2] 王静波[1] 马吉献[1] 

机构地区：[1]第四军医大学西京医院眼科全军眼科研究所,陕西西安710032 [2]解放军第273医院眼科,新疆库尔勒841000

出　　处：《西安交通大学学报（医学版）》2007年第2期215-219,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No:39970780)

摘　　要：目的观察重组人结缔组织生长因子(rhCTGF)对视网膜色素上皮(RPE)细胞增生和黏附的影响。方法用CTGF蛋白、多聚赖氨酸(PLL)和胶原分别包被培养板,观察CTGF促进RPE细胞黏附的作用;用四唑盐(MTT)比色试验和流式细胞仪(FCM)测定RPE细胞增生反应。结果10-30 mg/L的CTGF蛋白提高RPE细胞的贴壁黏附能力,与未包被的对照组相比,差异有统计学意义(P<0.05);30 mg/L CTGF促进RPE细胞黏附的能力与0.1 g/L胶原的作用一致,但略低于0.1 g/L PLL的作用。MTT实验显示CTGF刺激RPE细胞生长,CTGF作用24 h刺激RPE细胞增生的能力最强。FCM测定细胞周期结果显示S期百分比随着CTGF浓度增加而逐渐增加,无血清培养的对照组S期百分比为(7.5±0.4)%,60μg/LCTGF刺激24 h后,S期百分比上升至(16.1±2.0)%。结论CTGF可以促进RPE细胞的黏附和增生,在增生性玻璃体视网膜病变等眼内增生性疾病中可能有重要意义。Objective To investigate the effect of recombinant human connective tissue growth factor （rhCTGF） on adhesion and proliferation of retinal pigment epithelium （RPE） cells. Methods Microtiter plates of 96-wells were coated with CTGF, collagen protein and Poly-L-lysine （PLL）, respectively, to observe the effect of CTGF on RPE cell adhesion. Proliferation in RPE cells was evaluated using MTT assay and flow cytometry. Results 10-30 mg/L CTGF protein promoted the adhesion of RPE cells, which was significantly different when compared with the control（P〈0.05）. CTGF at 30 mg/L enhanced adhesion of RPE cells, and the effect was the same as that of collagen in 0.1 g/L, but less than that of PLL in 0.1 g/L. MTT assay showed that CTGF promoted the proliferation of RPE cells, and the maximal effect was observed at 24 hours by 60μg/L. FCM revealed that CTGF increased in the S phase cell percentage, and the S phase value in the control was （7.5 ± 0.4）% and （16.1± 2.0）% in 60μg/L CTGF. Conclusion rhCTGF stimulates adhesion and proliferation of RP, E cells, and CTGF may play an important role in the pathogenesis of intraocular proliferative diseases such as proliferative vitreoretinopathy.

关 键 词：结缔组织生长因子 视网膜色素上皮细胞 细胞黏附 细胞增生 

分 类 号：R774.1[医药卫生—眼科]