蛙皮素对PC-3细胞骨架及细胞内游离Ca^(2+)的影响  

Bombesin Increases Cytokeratin Expression and Free Calcium Concentration in PC-3 Cell Line

作　　者：武斌[1] 王禾[1] 秦卫军[1] 

机构地区：[1]第四军医大学西京医院泌尿外科,陕西西安710032

出　　处：《中华男科学杂志》2007年第4期332-334,共3页National Journal of Andrology

摘　　要：目的:观察蛙皮素(BBS)对前列腺癌PC-3细胞骨架形态及细胞内游离Ca2+([Ca2+]i)浓度的影响。方法:①利用免疫荧光法(IH),结合激光扫描共聚焦显微镜(LSCM)检测10-5mol/L浓度BBS处理的PC-3细胞角蛋白(CK)的表达,以反映其对细胞骨架形态的影响;②应用Fluo-3/AM荧光标记技术和LSCM检测不同浓度BBS(10-9、10-7、10-5mol/L)处理的PC-3细胞[Ca2+]i浓度。结果:①10-5mol/L浓度的BBS可促进PC-3细胞CK表达及伪足形成;②BBS可提高PC-3细胞[Ca2+]i浓度,并具有浓度依赖性。结论:实验证明一定浓度的BBS可明显提高PC-3细胞[Ca2+]i浓度及CK表达,进而影响PC-3细胞骨架形态。本研究为探索BBS应用于肿瘤研究以及BBS作用后细胞内信息传递途径提供了基础。Objective： To observe the effect of bombesin on cytoskeleton form and intracellular free calcium（ [ Ca^2＋ ] i） concentration in PC-3 prostate cancer cell line. Methods ： Immunofluorescent histochemistry （IH） combined with laser scanning confecal microscopy（LSCM） was used to examine the expression of cytokeratin（CK） in PC-3 cells treated with definite concentrations of BBS and observe its effect on cytoskeleton form. Fluo-3/AM fluorescence technique and LSCM were adopted to measure the [ Ca^2＋ ] i concentration after different concentrations （ 10 ^-9, 10 ^-7 and 10^-5mol/L） of BBS were added in PC-3 cells. Results： BBS（ 10 -Stool/L） stimulated the expression of CK in PC-3 cells and the formation of lamellipedium, and increased the [Ca^2＋ ]i concentration, with concentration dependence. Conclusion ： Definite concentrations of BBS could obviously enhance the [ Ca^2＋ ] i concentration, CK expression and cytoskeleton morphology of PC-3 cells. The results provide a basis for further studies on the role of BBS in turnout researches as well as in intracellular signal transmission.

关 键 词：前列腺癌 蛙皮素 激光共聚焦显微镜 荧光免疫测定 角蛋白 细胞内游离CA^2+ 

分 类 号：R737.25[医药卫生—肿瘤] R730.231[医药卫生—临床医学]