Inhibition of Tumor Growth in Mice by Endostatin Derived from Abdominal Transplanted Encapsulated Cells  

作　　者：Huaining TENG Ying ZHANG Wei WANG Xiaojun MA Jian FEI 

机构地区：[1]Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Model Organism Research Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [2]Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China [3]Department of Urology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China

出　　处：《Acta Biochimica et Biophysica Sinica》2007年第4期278-284,共7页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(30370447);the Major State Basic Research Development Program of China(2005CB522702);the Science and Technology Commission of Shanghai Municipality(03DZ14019,03DZ14018,03DJ14088,05DZ22915);Special Funds for Major State Basic Research of China(2002CB713803)

摘　　要：Endostatin, a C-terminal fragment of collagen 18a, inhibits the growth of established tumors and metastases in vivo by inhibiting angiogenesis. However, the purification procedures required for largescale production and the attendant cost of these processes, together with the low effectiveness in clinical tests, suggest that altemative delivery methods might be required for efficient therapeutic use of endostatin. In the present study, we transfected Chinese hamster ovary （CHO） cells with a human endostatin gene expression vector and encapsulated the CHO cells in alginate-poly-L-lysine microcapsules. The release of biologically active endostatin was confirmed using the chicken chorioallantoic membrane assay. The encapsulated endostatin-expressing CHO cells can inhibit the growth of primary tumors in a subcutaneous B 16 tumor model when injected into the abdominal cavity of mouse. These results widen the clinical application of the microencapsulated cell endostatin delivery system in cancer treatment.Endostatin, a C-terminal fragment of collagen 18a, inhibits the growth of established tumors and metastases in vivo by inhibiting angiogenesis. However, the purification procedures required for largescale production and the attendant cost of these processes, together with the low effectiveness in clinical tests, suggest that altemative delivery methods might be required for efficient therapeutic use of endostatin. In the present study, we transfected Chinese hamster ovary （CHO） cells with a human endostatin gene expression vector and encapsulated the CHO cells in alginate-poly-L-lysine microcapsules. The release of biologically active endostatin was confirmed using the chicken chorioallantoic membrane assay. The encapsulated endostatin-expressing CHO cells can inhibit the growth of primary tumors in a subcutaneous B 16 tumor model when injected into the abdominal cavity of mouse. These results widen the clinical application of the microencapsulated cell endostatin delivery system in cancer treatment.

关 键 词：MICROENCAPSULATION ENDOSTATIN CHO tumor gene therapy 

分 类 号：R730.54[医药卫生—肿瘤]