1,2,4-三氯苯矿化菌的鉴定与功能分析  

Identification of 1,2,4-Trichlorobenzene-Mineralizing Bacteria and Their Function Analysis

作　　者：王芳[1] U.Drfler M.Schmid S.Grundmann J.C.Munch 蒋新[1] R.Schroll 

机构地区：[1]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室 [2]Institute of Soil Ecology,GSF-National Research Center for Environment and Health,85764 Neuherberg,Germany

出　　处：《环境科学》2007年第5期1082-1087,共6页Environmental Science

基　　金：国家杰出青年科学基金项目(40325001);国家重点基础研究发展规划(973)项目(2002CB410805);中国科学院海外合作伙伴计划项目(CXTD-Z2005-4)

摘　　要：采用同位素示踪法从氯苯污染土壤中筛选到能高效降解1,2,4-三氯苯(1,2,4-TCB)为CO2的细菌E3和F2,根据其表型特征1、6S rDNA序列和荧光原位杂交(FISH)分析,鉴定E3和F2为博德特氏菌(Bordetellasp.).E3和F2的16S rDNA序列相似性为100%;它们与菌株Bordetellasp.QJ2-5同源性最高,与Bordetella petrii(GDH030510)最接近,同源性分别为100%和99.4%.E3和F2能在含1,2,4-TCB的无机盐培养基中彻底降解1,2,4-TCB生成CO2,30 d降解率达到90%以上,其中58%和46%矿化为CO2,同时形成一定量的微生物生物量.Two strains, E3 and F2, capable to mineralize 1,2,4-trichlorobenzene （1,2,4-TCB） were isolated from a chlorinated benzenes contaminated soil using ^14 C-1, 2,4-TCB as carbon source. They were identified by their 16S rDNA coding genes and fluoresence in situ hybridization （FISH） analysis as members of the genus Bordetella. A similarity of 100% were observed between strains E3 and F2 with their 16S rDNA sequences. They had the highest homology of 100% with Bordetella sp. QJ2-5 and the closest relation to described species, Bordetella petrii （GDH030510） with a similary of 99.4% . Strains E3 and F2 could degrade about 90% of 1,2,4-TCB and mineralize 58% and 46 % of 1 ,2,4-TCB to CO2 within 30 days in mineral liquid cultures, respectively. Biomass was formed during the mineralization process.

关 键 词：同位素示踪法 三氯苯 生物降解 博德特氏菌属(Bordetella sp.) 16S RDNA 荧光原位杂交(FISH) 

分 类 号：X172[环境科学与工程—环境科学]