乳酸乙酯对映体和丙酮酸乙酯混合物的气相色谱分析  

Gas Chromatographic Analysis of Mixture of Ethyl Lactate Enantiomer and Ethyl Pyruvate

作　　者：陶国忠[1] 郭耘[1] 卢冠忠[1] 王艳芹[1] 郭杨龙[1] 张志刚[1] 王筠松[1] 刘晓晖[1] 

机构地区：[1]华东理工大学工业催化研究所结构可控先进功能材料及其制备教育部重点实验室,上海200237

出　　处：《分析化学》2007年第3期447-450,共4页Chinese Journal of Analytical Chemistry

基　　金：上海市科委纳米专项基金资助(No.0552nm022)

摘　　要：运用气相色谱手性固定相在适宜的色谱操作条件下将丙酮酸乙酯和乳酸乙酯手性对映体完全分离。以环戊酮为内标物定量测定了丙酮酸乙酯和乳酸乙酯手性对映体的含量,测试结果的相对误差范围为-1.66%^+1.23%,回收率范围为97.03%~101.9%;RSD为0.15%~0.86%。该法具有简捷、高效、准确、稳定性强、重复性好及线性范围宽等特点,是一种可以同时定量测定丙酮酸乙酯、R-乳酸乙酯及S-乳酸乙酯含量的方法。Mixture of chiral enantiomers of ethyl lactate and ethyl pyruvate was separated completely by gas chromatography on chiral stationary phase. The optimized operating parameters were as follows： column temperature 90℃ ; detector temperature 180℃ ; injector temperature 200℃ ; split ratio 1 ： 60; inection volume 0. 30 μL; carrier gas pressure 0.04 MPa; H2 pressure 0.10 MPa; air pressure 0. 030 MPa, and the retention times of R-,S-ethyl lactate, ethyl pyruvate were 13.0 rain, 14.02 min, 7.96 min, respectively. The contents of R, S-ethyl lactate enantiomer and ethyl pyruvate were quantitatively determined by using cyclopentanone as an internal standard with relative correction factor 2. 145, 2. 139, 2. 805, respectively. The results show that this method with relative error of - 1.7% - ＋ 1.2% , recovery of 97.03% - 101.9% and relative standard deviation of 0.2% - 0.9% is simple, highly efficient, accurate, stable, well repeatable and widely linear for determining the content of ethyl pyruvate, R-and S-ethyl lactate in the mixture solution by GC and internal standard method.

关 键 词：气相色谱分析 乳酸乙酯 丙酮酸乙酯 对映体纯度 环戊酮内标法 

分 类 号：O657.71[理学—分析化学]