紫菜多糖对小鼠NK细胞活性的增强作用  

Immune-reinforcing effects induced by Porphyra polysaccharide on natural killer cell activity of mice

作　　者：李胜军[1] 吕昌龙[1] 李成[1] 曹妍[1] 闫建中[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,辽宁沈阳110001

出　　处：《中国医科大学学报》2007年第2期133-134,共2页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20022065)

摘　　要：目的:探讨紫菜多糖对小鼠免疫功能的刺激作用。方法:以不同浓度的紫菜多糖及生理盐水向BALB/C小鼠腹腔连续注射7d后,通过ELISA技术及乳酸脱氢酶释放法分别检测小鼠的脾细胞分泌γ-干扰素(IFN-γ)水平及自然杀伤细胞(NK细胞)杀伤活性。结果:0.15g·kg-1紫菜多糖注射组小鼠的脾细胞分泌IFN-γ水平及NK细胞杀伤活性高于生理盐水对照组(P<0.05)。结论:适宜浓度的紫菜多糖具有增强小鼠免疫功能的效应。Objective：To investigate the immune-reinforcing effect of Porphyra polysaccharide in mice. Methods： After intraperitoneal injection of different concentratons of Porphyra polysacchaxide or normal saline for 7 days, the levels of interferon-γ （IFN-γ） produced by spleen cells and the activity of natural killer （NK）cells in BALB/C mice were determined by enzyme-linked immunosorbent assay and lactic dehydrogenase release reaction, respectively. Results： The levels of IFN-γ produced by spleen cells and the activity of NK cells in mice injected with 0.15 g/kg of Porphyra polysaccharide were higher than those in mice injected with normal saline. Conclusion： Appropriate concentration of Porphyra polysaccharide can reinforce immune function in mice.

关 键 词：紫菜多糖 Γ-干扰素 自然杀伤细胞 免疫调节 

分 类 号：R931.77[医药卫生—生药学] R967[医药卫生—药学]