Ets-1和VEGF在实验性糖尿病视网膜中的共表达(英文)  

Concomitant expression and combined localization of Ets-1 and VEGF in the experimental diabetic rat's retina

作　　者：杜兆江[1] 李鹏[2] 惠延年[1] 王百忍[3] 段小莉[3] 张瑞[4] 

机构地区：[1]第四军医大学西京医院眼科,中国陕西省西安市710032 [2]解放军第451医院眼科,中国陕西省西安市710054 [3]第四军医大学全军神经生物研究所,中国陕西省西安市710032 [4]第四军医大学唐都医院药剂科,中国陕西省西安市710038

出　　处：《国际眼科杂志》2007年第2期294-297,共4页International Eye Science

摘　　要：目的:阐明VEGF和Ets-1在糖尿病大鼠视网膜内的表达特点和分布特点。方法:腹腔注射STZ诱导糖尿病大鼠模型。STZ注射后4wk处死动物,分离各组视网膜总蛋白进行Western blot分析。将眼球固定后制成14μm厚的冰冻切片进行Ets-1和VEGF免疫荧光双标检测。结果:Ets-1和VEGF在糖尿病大鼠视网膜内表达均显著增强,而且Ets-1和VEGF在视网膜内的分布特点相似,几乎在视网膜各层均共表达。结论:Ets-1可能对VEGF诱导的糖尿病视网膜病变有促进作用。AIM： To investigate the characteristics of Ets-1 and VEGF expression and distribution in the experimental diabetic rat retina. METHODS： Diabetes was induced by intraperitoneal injection of streptozotocin （STZ）. At 4 weeks after STZ-injection, animals were sacrificed. Total proteins were isolated from retinas of experimental and control eyes and were assessed by Western blot analysis. Frozen cross sections of eyeballs with 24um thickness were used to perform double immunofluores-cence staining with anti-Ets-1 and anti-VEGF antibodies. RESULTS： Both Ets-1 and VEGF expression were up-regulated in the diabetic retina, the distribution of Ets-1 and VEGF was identical to each other, and the two proteins were almost localized in all retinal layers. CONCLUSION： Ets-1 might contribute to the pathologic progress of the diabetic retina induced by VEGF.

关 键 词：ETS-1 VEGF 糖尿病视网膜病变 血管发生 

分 类 号：R774.1[医药卫生—眼科]