吡喃糖氧化酶底物特异性研究  

A Study of Substrate Specificity on Pyranose Oxidase

作　　者：李玉运[1] 张孝山[2] 樊晶[2] 李立新[2] 

机构地区：[1]天津市血液中心输血研究所,300110 [2]天津市血液中心

出　　处：《天津医药》2007年第4期272-274,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金面上资助项目(项目编号:013609611)

摘　　要：目的:评价吡喃糖氧化酶(PROD)对不同的糖底物、甘油和乙二醇的催化作用。方法:应用相同酶试剂和固定参数对12种糖、甘油和乙二醇进行催化率实验。结果:将其对葡萄糖的催化作用定为100%时,PROD对单糖中的山梨糖催化率最高(87.51%),其次是木糖(83.17%)和1,5-脱水-D-山梨醇(82.32%),半乳糖为40.69%,对果糖、甘梨醇、山梨醇几乎无催化作用;PROD对双糖中的麦芽糖催化率为3.19%,对乳糖、蔗糖、纤维二糖几乎无催化作用;对同等浓度的非糖类物质甘油和乙二醇的催化作用很小,但是对高浓度的甘油和乙二醇(应用浓度在2.2%～3.3%)催化活性很高。结论:建立1,5-脱水-D-山梨醇测定方法前应进行PROD对不同糖底物、甘油和乙二醇的特异性催化实验。Objective： To evaluate the catalyzing effects of pyranose oxidase （PROD） on different sugar substrates, glycerol and ethylene glycol. Methods： The same working enzymatic reagents were used for evaluating the catalyzing specificity to all 12 sugar substrates, glycerol and ethylene glycol with the same test parameters. Results： The effect of catalyzing for glucose was de- termined as 100%. The PROD reagent had marked catalyzing effects for L-sorbose （87.51%）, D-xylose （83.17%）, 1,5-anhydro- D-glucitol （82.32%） and D-galactose （40.69%）. There was little effect for D-fructose, mannitol and sorbitol in monosaccharides. The PROD had catalyzing effects for maltose（3.19%） but little effect for a-lactose, sucrose and cellobiose. The PROD had a little effect for glycerol and ethylene glycol with the same concentration but higher catalyzing effects for high concentration of glycerol and ethylene glycol （2.2%-3.3%）. Conclusion： Before a new assay is set up the catalyzing specificity of the enzyme for its substrate should be evaluated.

关 键 词：氧化还原酶类 葡萄糖 山梨醇 底物特异性 

分 类 号：R446.112[医药卫生—诊断学]