幽门螺杆菌与胃黏膜bFGF，FGFR-2表达的关系及意义  

Correlations of Helicobacter pylori infection with the expression of basic fibroblast growth factor and fibroblast growth factor receptor-2 in gastric mucosa and their significances

作　　者：李信[1] 姜海行[1] 陈罡[2] 雷琳[1] 覃山羽[1] 

机构地区：[1]广西医科大学第一附属医院消化内科,广西壮族自治区南宁市530021 [2]广西医科大学病理学教研室,广西壮族自治区南宁市530021

出　　处：《世界华人消化杂志》2007年第9期964-969,共6页World Chinese Journal of Digestology

摘　　要：目的：探讨幽门螺杆菌（H pylori）感染的胃黏膜上皮细胞碱性成纤维细胞生长因子（bFGF）、成纤维细胞生长因子受体-2（FGFR-2）的表达及其在胃黏膜癌变过程中的意义.方法：选择慢性浅表性胃炎（CSG）30例、胃黏膜肠上皮化生（IM）29例、不典型增生（Dys）31例及胃癌（GC）55例.采用免疫组化SP法检测胃黏膜上皮细胞bFGF,FGFR-2表达状况,用快速尿素酶试验和组织学Warthin-Starry嗜银染色法联合检测胃黏膜Hpylori感染情况.结果：CSG组bFGF,FGFR-2的表达显著低于其余三组（IM组：x^2=4.002,P＜0.05;x^2=4.163,P＜0.05;Dys组：x^2=15.779,P=0.000;x^2=15.949,P=0.000;GC组：x^2=24.110,P=0.000;x^2=18.736,P=0.000）,IM组的表达低于Dys组及GC组（Dys组：x^2=4.258,P＜0.05;x^2=4.212,P＜0.05;GC组：x^2=7.786,P＜0.01;x^2=4.687,P＜0.05）,而Dys组与GC组间无显著性差异.Hpylori阳性IM及Dys组bFGF, FGFR-2的表达均显著高于阴性组（IM组：x^2=10.076,P＜0.01;x^2=7.535,P＜0.01;Dys组：x^2=11.501,P＜0.01;x^2=8.330,P＜0.01）.Hpylori阳性Dys组bFGF, FGFR-2表达显著高于GC组（x^2=4.201,P＜0.05;x^2=3.982,P＜0.05）,H pylori阳性IM组则与G C组的表达无显著性差异;Hpylori阴性Dys组及IM组bFGF的表达均显著低于GG组（x^2=5.736,P＜0.05;x^2=17.113,P=0.000）,H pylori阴性Dys组FGFR-2表达与GC组无显著性差异而IM组的FGFR-2的表达显著低于GC组（x^2=11.091,P＜0.05）.结论：H pylori感染引起胃黏膜上皮细胞bFGF及FGFR-2的过度表达可能与H pylori感染致胃黏膜上皮细胞的癌变有关.AIM： To study the expression of basic fibroblast growth factor （bFGF） and fibroblast growth factor receptor-2 （FGFR-2） in gastric mucosal epithelia with H pylori infection and their significances in the carcinogenesis of stomach cancer. METHODS： A total of 30 patients with chronic superficial gastritis （CGS）, 29 with intestinal metaplasia （IM）, 31 with dysplasia （Dys） and 55 with gastric cancer （GC） were included in this study. The expression of bFGF and FGFR-2 were assessed by immunohistochemistry （SP method）. Rapid urease test and histological examination with Warthin-Starry were used to determine the status of H pylori infection. RESULTS： The expression of bFGF and FGFR-2 in CSG patients were significantly lower than those in the other patients （IM： x^2 = 4.002, P 〈 0.05; x^2 = 4.163, P 〈 0.05; Dys：x^2 = 15.779, P = 0.000; x^2 = 15.949, P = 0.000; GC： x^2 = 24.110, P = 0.000;x^2 = 18.736, P = 0.000）. The expression of bFGF and FGFR-2 in IM patients were significantly lower than those in Dys and GC patients （Dys： x^2 = 4.258, P 〈 0.05; x^2 = 4.212, P 〈 0.05; GC： x^2 = 7.786, P 〈 0.01; x^2 = 4.687, P 〈 0.05）. But there was no significant difference between Dys and GC patients. The positive rates of bFGF and FGFR-2 expression were significantly higher in H pylori-infected IM and Dys patients than those in the patients without H pylori infection （IM： x^2 = 10.076, P 〈 0.01;x^2 = 7.535, P 〈 0.01; Dys：x^2 = 11.501, P 〈 0.01; x^2 = 8.330, P 〈 0.01）. The positive rates of bFGF and FGFR-2 expression in H pylori-infected Dys patients were significantly higher than those in GC group （bFGF：x^2 = 4.201, P 〈 0.05; FGFR-2：x^2 = 3.982, P 〈 0.05）, while no significant difference was found between GC and IM patients. The expression of bFGF in Dys and IM patients without H pylori infection were significantly lower than that in GC patients （Dys： x^2 = 5.736, P 〈 0.05; IM：x^2 = 17.113, P = 0.000）, and FGFR-2 expression in H py

关 键 词：幽门螺杆菌 碱性成纤维细胞生长因子 成纤维细胞生长因子受体-2 胃黏膜 癌变 免疫组织化学 

分 类 号：R573[医药卫生—消化系统]