血管紧张素Ⅱ对肝星状细胞迁移和增殖的影响  

Effect of angiotensinⅡon the proliferation and migration of hepatic stellate cells

作　　者：杨玲[1] 朱清静[2] 侯晓华[1] 胡胜军[1] 徐敏[1] 朱锐[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院消化内科,湖北省武汉市430022 [2]湖北省中医院肝病中心,湖北省武汉市430061

出　　处：《世界华人消化杂志》2007年第9期1004-1007,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金青年基金;No.30500658;中国博士后科学基金;No.2004035198;湖北省自然科学基金;No.2004ABA236

摘　　要：目的:探讨血管紧张素Ⅱ(ANGⅡ)对肝星状细胞(HSC)增殖、迁移活性的影响.方法:体外培养HSC,在不同浓度的ANGⅡ作用下,观察HSCs生长情况并绘制生长曲线,采用MTT法测定细胞增殖、Transwell小室检测细胞迁移.结果:1,2,4,8,10 umol/L ANGⅡ可显著促进HSC增殖,与空白对照组相比有显著差异(F= 2.305,P<0.05;F=4.003,6.833,8.855,21.066,P<0.01).随作用浓度的增加,细胞增殖活性显著提高,相关分析显示呈正相关(r=0.917,P<0.01).10,8,4μmol/L的ANGⅡ可显著诱导HSC迁移,与空白对照组相比差异显著(F= 22.084,15.155,10.392,P<0.01).随ANGⅡ浓度的增加,细胞迁移率显著升高,相关分析显示呈正相关(r=0.952,P<0.01).结论:ANGⅡ可显著诱导HSC增殖与迁移,并随剂量的增加作用加强.AIM： To explore the changes of the proliferation and migration ability of hepatic stellate cells （HSCs） induced by angiotensin Ⅱ （ANG Ⅱ ）. METHODS： HSCs were incubated in vitro. The proliferation and migration ability of HSCs induced by ANG Ⅱ at different concentration were observed with MTT assay and Transwell chamber assay, respectively. RESULTS： The proliferation of HSCs was significantly increased when ANG Ⅱ was used at the concentrations of 1, 2, 4, 8 and 10 μmol/L in comparison with that in the control cells （F = 2.305, P 〈 0.05; F = 4.003, 6.833, 8.855, 21.066, P 〈 0.01）. With the increasing of ANG Ⅱ concentration, the proliferative activity was increased, showing a positive correlation （r = 0.917, P 〈 0.01）. ANG Ⅱ at the concentrations of 10, 8 and 4 μmol/L markedly induced the migration of HSCs （F = 22.084, 15.155, 10.392, P 〈 0.01）. The migration ability of HSCs was also positively correlated with the concentrations of ANG Ⅱ （r = 0.952, P 〈 0.01）. CONCLUSION： ANG Ⅱ can significantly induce the proliferation and migration of HSCs in a dose-dependent manner.

关 键 词：血管紧张素Ⅱ 肝星状细胞 细胞迁移 增殖 MTT法 

分 类 号：R575.2[医药卫生—消化系统]