葡萄糖神经酰胺合成酶基因在人胃癌细胞SGC-7901及SGC-7901/VCR的表达和意义  

Expression of glucosylceramide synthase gene and its significance in human gastric carcinoma SGC-7901 and SGC-7901/VCR cells

作　　者：张艳[1] 刘宗文[2] 贾丹辉[1] 乐晓萍[3] 张钦宪[3] 

机构地区：[1]郑州大学基础医学院药理学教研室,河南省郑州市450052 [2]河南省肿瘤病理重点实验室,河南省郑州市450052 [3]郑州大学基础医学院组织胚胎学教研室,河南省郑州市450052

出　　处：《世界华人消化杂志》2007年第9期1008-1011,共4页World Chinese Journal of Digestology

摘　　要：目的:研究葡萄糖神经酰胺合成酶(glucosyl- ceramide synthase,GCS)基因在人胃癌细胞SGC-7901和SGC-7901/VCR的表达并探讨其对人胃癌发生、发展的意义.方法:体外培养人亲本敏感胃癌细胞SGC-7901和人耐长春新碱胃癌细胞SGC-7901/VCR.采用MTT法检测半数细胞抑制浓度(IC_(50))和SGC-7901/VCR耐药倍数;RT- PCR法检测SGC-7901和SGC-7901/VCR两种细胞GCS mRNA的表达,免疫组化法测定GCS蛋白表达水平.结果:SGC-7901和SGC-7901/VCR的IC_(50)分别为1.40±0.06和86.20±0.50 mg/L.SGC-7091/ VCR细胞耐药指数是亲本细胞SGC-7901的61倍,SGC-7901和SGC-7901/VCR两种细胞均有GCS mRNA表达,且SGC-7901/VCR细胞GCS mRNA表达水平(GCS指数为3.9)较SGC-7901细胞(GCS指数为0.5)有显著升高(P<0.05),耐药细胞GCS蛋白表达阳性率(65%)显著高于亲本细胞(18%)(P<0.05).结论:GCS基因在人胃癌耐药细胞和亲本敏感细胞均有表达,耐药细胞GCS mRNA及蛋白均高表达.GCS可能参与了胃癌的发生过程,且与肿瘤多药耐药有密切关系.AIM： To investigate the expression of glucosylceramde synthase （GCS） gene and its significance in human gastric carcinoma SGC-7901 and SGC-7901/VCR cells. METHODS： Human gastric carcinoma SGC-7901/VCR and SGC-7901 cells were cultured in vitro, and the total RNA was extracted by TRIzol method. MTr was adopted to evaluate the drug resistance of SGC-7901/VCR cells. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expression of GCS mRNA, and Western blotting and immunocytochemistry were performed to measure the expression of GCS protein. RESULTS： The half inhibitory concentrations （IC50） for SGC-7901 and SGC-7901/VCR were 1.40 ± 0.06 and 86.20 ± 0.50 mg/L, respectively, and the drug-resistant index of SGC-7901/VCR cells was 61 times as high as that of SGC-7901 cells. GCS mRNA was expressed in both SGC-7901/VCR and SGC-7901 cells, and there was significant difference between them （GCS index： 3.9 vs 0.5, P 〈 0.05）. Moreover, GCS protein expression was significantly different between SGC-7901/VCR and SGC-7901 cells （65% vs 18%, P 〈 0.05）. CONCLUSION： GCS gene expresses in human gastric cancer cells and it plays an important role in the carcinogenesis and development of gastric carcinoma. The high expression of GCS is closely related with the multidrug resistance of tumors.

关 键 词：人胃癌细胞 葡萄糖神经酰胺合成酶 基因表达 多药耐药 

分 类 号：R735.2[医药卫生—肿瘤]