异体角膜缘上皮细胞培养后移植的实验研究  

The experimental studies for limbal epithelial cells culture and allogeneic transplantation

作　　者：刘兴华[1] 朱美玲[1] 陈逖[1] 王明丽[1] 

机构地区：[1]安徽医科大学第一附属医院眼科,合肥230022

出　　处：《临床眼科杂志》2007年第2期169-171,共3页Journal of Clinical Ophthalmology

基　　金：安徽省教育厅基金资助项目(编号:2000jl103)

摘　　要：目的观察含培养的角膜缘上皮细胞的羊膜片移植到角膜缘干细胞损伤的异体兔眼角膜上的效果。方法把羊膜为载体组织块培养的角膜缘上皮细胞移植到角膜缘干细胞损伤的异体兔眼角膜上,术后观察角膜混浊度、角膜新生血管、角膜上皮等情况,定期兔进行病理组织学检查。结果角膜缘上皮细胞在羊膜上培养16d形成3～4层,用含有培养的异体角膜上皮细胞的羊膜片移植的12只兔眼,术后第5天,损伤的角膜表面全部上皮化,第16天开始,12只兔眼逐渐出现排斥反应。结论含培养的角膜缘上皮细胞羊膜片异体移植术后早期角膜全部上皮化,晚期则出现排斥反应。Objective To observe the effect of amniotic membrane containing cultivated limbal epithelial ceils which were transplanted to allogeneic limbal stem cell damaged rabbit cornea. Methods Cultivated limbal epithelial ceils by tissue inoculation on amniotic membrane, were transplanted allogeneic limbal stem cell damage rabbit cornea. Outcome （corneal opacity, vascularization,the process of corneal rcepithelialization） was assessed by daily slit-lamp examination, and histopathologic examination was carried out regularly. Results Experimental group entire corneal were all successfully epithelialied up to 5 days after surgery while control group rabbits eyes of corneal epithelia remained full defected. From the 16th day, experimental group rabbits eyes appeared to undergo rejection while control group not. Conclusion All experimental group corneas were all epithelialized in the earlier postoperative period and were undergone rejection in the later postoperative period.

关 键 词：角膜缘上皮细胞 组织块培养 羊膜 异体移植 排斥反应 

分 类 号：R77[医药卫生—眼科]