草药制剂STW-5-NII对大鼠小肠传入神经电活动的影响  

The effect of a new herbal STW-5-N-II on intestinal afferent nerve discharge in rat

作　　者：巩倩[1] 李永渝[1] 

机构地区：[1]同济大学病理生理学教研室,上海200092

出　　处：《同济大学学报（医学版）》2007年第2期28-33,共6页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金资助项目(30370643)

摘　　要：目的探讨不同浓度草药复方制剂STW-5-NII对不同化学物及机械刺激诱导的大鼠小肠传入神经电活动变化的影响,并分析其可能的机制。方法24只雄性Wister大鼠均分为4组,麻醉后胃管灌入不同浓度STW-5-NII药液(原液、50%原液、25%原液)或对照液预处理,用细胞外多元肠系膜传入神经记录技术(extracellular multi-unitmesenteric afferent nerve recording)描记肠传入神经元电活动信号。静脉注射不同剂量5-羟色胺(5-HT)(5、10、20、40μg/kg)或不同剂量缓激肽(BK)(15、30、60μg/kg),或给与不同程度的肠管扩张(0 cmH2O^60 cmH2O)刺激,观察各种刺激前后小肠传入神经电活动的变化,比较和分析不同浓度STW-5-NII对此变化的影响。结果不同剂量的5-HT和BK均可引起小肠传入神经电活动的增加;STW-5-NII可明显降低BK的增加作用(P<0.05,或P<0.01),对5-HT诱导的小肠传入神经电活动的增加STW-5-NII有降低的趋势,但差异无显著性(P>0.05);肠管扩张刺激时随着肠腔内压力的升高小肠传入神经电活动增加,不同浓度的STW-5-NII对此也有降低作用,但差异无显著性(P>0.05)。结论STW-5-NII可以降低大鼠小肠对BK的敏感性,这为该药用于治疗一些与内脏敏感性增高有关的胃肠道运动功能障碍性疾病提供了实验依据。Objective To investigate the effect of different concentrations of a new simplified herbal combination product STW-5-NII on intestinal afferent nerve discharge under different mechanical and chemical stimuli. Methods Twenty-four Wister Rats were divided into four groups. STW-5-NII at a concentration of 100%, 50 %, 25 % or vehicle were administered orally in rats after anesthetized. The extracellular multi-unit mesenteric afferent nerve recordings were obtained in the proximal jejunum. Afferent discharge to 5-Hydroxytryptamine （5-HT） （5,10,20 and 40 μg/kg） , bradykinin（BK） （15,30 and 60 μg/kg） and luminal distension （060 cmH2O）were recorded and analyzed. Results Afferent nerve discharge was increased after different dose of 5- HT and BK. Afferent nerve sensitivity to bradykinin but not to 5-HT was significantly different depending on the dose of pretreatment with STW-5-NII. A pressure-dependem increase in afferent nerve discharge was observed during intraluminal distension that was not significantly different following pretreatment with any concentraction of STW-5-NII. Conclusion The herbal preparation STW-5-NII desensitizes intestinal afferents selectively to systemic bradykinin. This new preparation might be helpful for patients suffering from disorders associated with visceral hypersensitivity.

关 键 词：小肠传入神经 胃肠道运动 草药 STW-5-NII 大鼠 

分 类 号：R576[医药卫生—消化系统] R975[医药卫生—内科学]